摘要
【目的】探究ε-聚赖氨酸(ε-PL)产生菌对p H和ε-PL的耐受性、氧化胁迫与ε-PL合成之间的关系。【方法】选取3株ε-PL产生菌Streptomyces sp.AF3-44、Streptomyces sp.AS32和Streptomyces albulus F15,比较其在发酵性能、p H和ε-PL耐受性以及抗氧化胁迫能力上的差异,并对菌株发酵过程的活性氧成因进行分析。【结果】在3株菌中AF3-44具有最强的p H和ε-PL耐受性及抗氧化胁迫能力,因而在发酵后期能够保持良好的细胞活性和最高的ε-PL浓度;ε-PL引起的氧化胁迫主要发生在发酵前期,而发酵中后期氧化胁迫的产生主要由酸性p H导致。【结论】提高链霉菌ε-PL发酵过程中的抗氧化胁迫能力,可提升菌体活力和发酵水平。
[Objective] To study the relationship between the tolerance of p H and ε-PL, oxidative stress and ε-poly-L-lysine（ε-PL） synthesis in ε-PL producing strain. [Methods] The fermentation activities, p H and ε-PL tolerance, and antioxidative abilities among Streptomyces sp. AF3-44, Streptomyces sp. AS32 and Streptomyces albulus F15 were compared, and the cause for reactive oxygen species generation was analyzed. [Results] AF3-44 had the best p H and ε-PL tolerance and antioxidative ability among three tested strains, and kept good cell activity with the highest ε-PL concentration. ε-PL caused the oxidative stress at the earlier but p H at the later stage of fermentation. [Conclusion] Improvement of the antioxidative ability in ε-PL fermentation by Streptomyces could increase the cell activity and ε-PL level in fermentation.
出处
《微生物学通报》
CAS
CSCD
北大核心
2017年第11期2547-2556,共10页
Microbiology China
基金
国家自然科学基金项目(No.21376106)
111引智计划(No.111-2-06)~~
关键词
氧化胁迫
Ε-聚赖氨酸
活性氧
链霉菌
Oxidative stress, ε-Poly-L-lysine, Reactive oxygen species, Streptomyces
