摘要
研究NaCl胁迫对夏枯草幼苗抗氧化能力与光合特性的影响,为夏枯草栽培管理、抗盐育种提供理论依据。以夏枯草幼苗为材料,采用营养液培养的方法研究不同浓度NaCl(0、20、40、60、80mmol/L)胁迫对夏枯草幼苗抗氧化酶、渗透调节物质、叶绿素、光合特性的影响。结果表明,随着盐胁迫浓度的升高,夏枯草叶片的超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、可溶性蛋白含量呈先上升后下降的趋势;过氧化物酶(POD)、可溶性糖含量、丙二醛(MDA)含量与电导率呈不断升高的趋势;叶绿素a和b、叶绿素a/b、类胡萝卜素及总叶绿素均呈逐渐下降的趋势;净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)均表现下降的趋势,胞间二氧化碳浓度(Ci)则表现为先下降后上升的趋势,而气孔限制值(Ls)表现为先升高后降低的趋势。在20~60mmol/L NaCl处理下,夏枯草叶片光合速率的下降主要是由气孔限制引起的,在80mmol/L NaCl处理下叶片光合速率的下降则主要是由非气孔限制引起的。NaCl胁迫下,夏枯草通过提高抗氧化酶的活性、渗透调节物质的含量来保护光合机构,从而提高夏枯草幼苗对盐胁迫的适应性。
The effects of salt stress on antioxidant capacity and photosynthetic characteristics of Prunella vulgaris seedlings were studied to provide a theoretical understanding to aid cultivation and management,and the breeding of salt resistant varieties of P.vulgaris.P.vulgaris seedlings were incubated in nutrient solution,the seedlings were treated with NaCl concentrations of 0,20,40,60 and 80 mmol/L.Antioxidant enzyme activities,osmolyte concentrations,chlorophyll content,and photosynthesis parameters were quantified after the treatment.The results showed that with increasing salt concentration,superoxide dismutase,ascorbate peroxidase,and soluble protein content increased with increasing salt concentration to 40 or 60 mmol/L and then decreased as concentration rose further;peroxidase,soluble sugar content,malondialdehyde content and electric conductivity showed a continuously rising trend;chlorophyll a and chlorophyll b,chlorophyll a/b,carotenoid and total chlorophyll declined with every increase in salt concentration,as did net photosynthetic rate,transpiration rate and stomatal conductance.Intercellular CO2 concentration first decreased as NaCl concentration increased,with the lowest value at 60 mmol/L salt concentration and the highest at 80 mmol/L NaCl.The stomatal limit value steadily increased from 0.26 in CK to 0.38 in 60 mmol/L NaCl,and then decreased to 0.24 at80 mmol/L.The decrease in net photosynthetic rate caused by 20-60 mmol/L NaCl stress was considered to be a result of stomatal restriction,the decrease of net photosynthetic rate caused by 80 mmol/L NaCl stress was mainly attributable to non-stomatal limitation.Under NaCl stress,P.vulgaris increased the activity of antioxidant enzymes and the contents of osmolytes,and thus increased salt stress tolerance.
出处
《草业学报》
CSCD
北大核心
2017年第11期167-175,共9页
Acta Prataculturae Sinica
基金
河南省科技攻关计划项目(162102110095)
河南科技大学学科提升振兴A计划项目(13660001)
河南科技大学高级别项目培育基金(2015GJB029)
河南科技大学博士科研启动基金资助项目(4024-13480054
4026-13480038)
河南科技大学大学生研究训练计划(SRTP)项目(2014254
2014281
2015152
2016136)资助
河南省高等学校重点科研资助项目(15A180037
16A220005)
关键词
夏枯草
NACL胁迫
光合
抗氧化酶
Prunella vulgaris
NaCl stress
photosynthesis
antioxidant enzyme
