摘要
背景:关节软骨损伤多由创伤及骨关节炎引起,其治疗是临床研究的难点和热点。组织工程技术的发展为修复关节软骨缺损提供了新方法,但软骨细胞来源不足一直是一个难题。目的:探讨软骨细胞及骨髓间充质干细胞共培养修复关节软骨缺损的可行性,评价修复效果。方法:体外培养扩增猪骨髓间充质干细胞及关节软骨细胞,共培养2代,接种于聚乳酸-聚羟基乙酸聚合物上继续共培养2周,构建共培养组织工程软骨(共培养组);以单纯软骨细胞构建组织工程软骨为对照组,回植修复关节软骨缺损;空白组软骨缺损不作处理。半年后行大体观察及组织学观察,定量检测Ⅱ型胶原染色面积及氨基糖胺聚糖含量。结果与结论:(1)共培养组的修复组织呈软骨样,表面光滑平坦,与周围关节软骨组织整合良好;对照组呈纤维组织修复;而空白组则无修复;(2)组织学观察显示,共培养组结构致密,细胞外基质分布更均匀,与周围关节软骨组织及软骨下骨组织整合良好;(3)共培养组的Ⅱ型胶原染色面积及糖胺聚糖含量均优于对照组及空白组,差异有显著性意义(P<0.05);(4)综上,软骨细胞和骨髓间充质干细胞共培养构建组织工程软骨能有效修复关节软骨缺损。
BACKGROUND： Articular cartilage damage caused by traumatic articular cartilage defects and osteoarthritis is acommon clinical disorder, and its treatment is an issue of concern. Tissue engineering provides a new method forarticular cartilage repair. But insufficient cartilage cell source is always a thorny issue.OBJECTIVE： To explore the feasibility of repairing articular cartilage defects with tissue-engineered cartilageconstructed by co-culture of chondrocytes and bone marrow mesenchymal stem cells （BMSCs）, and evaluate thecurative efficacy.METHODS： Chondrocytes and BMSCs were isolated from swine articular cartilages, expanded in vitro, mixed after thefirst passage, and then co-cultured for another passage. The mixed cells were seeded onto a polyglycolic acid/polylacticacid scaffold and, followed by 2 weeks co-culture, then sutured into osteochondral complex. The co-culturetissue-engineered cartilage was transplanted into the defect region. The tissue-engineered cartilage constructed withsingle chondrocytes served as control group. Those received no intervention as blank control group. Six months later,the gross observation and histological staining were performed, as well as the dying area of collagen type II and level ofglucosamine polysaccharide were detected.RESULTS AND CONCLUSION： In the co-culture group, the tissues were chondroid with smooth and glossy surface,and well connected with the surrounding tissues. The control group presented with fiber-like tissues, while the blankcontrol group showed no changes. The distribution of cartilaginous extracellular matrice in the co-culture group was morehomogenous than the others, and there was a good connection between newly born tissues and the surrounding tissuesas well as subchondral bone. Furthermore, the dying area of collagen type II and level of glucosamine polysaccharide inthe co-culture group were significantly higher than those in the other groups （P 〈 0.05）. To conclude, the co-culture ofchondrocytes and BMSCs can improve the quality of tissue-engineered cartilage, which effectively contributes to therepair of articular cartilage.Subject headings： Chondrocytes; Mesenchymal Stem Cells; Cartilage, Articular; Tissue Engineering.
出处
《中国组织工程研究》
CAS
北大核心
2017年第32期5122-5127,共6页
Chinese Journal of Tissue Engineering Research
基金
南京医科大学科技发展基金项目(2013NJMU182)~~
