CXC型趋化因子配体12/CXC趋化因子受体4在非小细胞肺癌组织的表达被引量：1

Expression of CXC chemokine ligand 12 and CXC chemokine receptor 4 in non - small cell lung cancer tissues and clinical implication

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摘要目的检测CXC型趋化因子配体12（CXCL12）、CXC趋化因子受体4（CXCR4）在非小细胞肺癌组织及癌旁正常肺组织及转移淋巴结中的表达，分析其阳性表达的意义，探讨非小细胞肺癌（NSCLC）淋巴结转移的相关性。方法采用免疫组织化学染色方法检测62例NSCLC患者癌组织和匹配的癌旁正常肺组织中CXCL12、CXCR4的表达，切片在显微镜下观察10个高倍视野计数，阳性细胞数≥10%为阳性（＋），阳性数小于10%为阴性（－）。分析其表达率与各种临床参数之间的关系。结果NSCLC细胞CXCL12的阳性表达率73.8%，NSCLC癌旁正常肺组织阳性表达率15.0%，两者差异有统计学意义。NSCLC细胞CXCR4的阳性表达33例，NSCLC癌旁正常肺组织阳性表达2例，两者差异有统计学意义。CXCL12表达阳性率在Ⅲa-Ⅲb期为90.9%，在Ⅱa-Ⅱb期为55.5%；CXCR4表达阳性率在Ⅲa-Ⅲb期为90.9%，在Ⅱa-Ⅱb期为55.5%（P＝0.030）其表达差异均有统计学意义。CXCL12的阳性表达率在纵隔淋巴结转移阳性为94.7%，在纵隔淋巴结转移阴性为56.5%。CXCL12、CXCR4在NSCLC组织中均一致表达。结论CXCL12、CXCR4在NSCLC组织中表达具有促癌作用。CXCL12、CXCR4的阳性表达与TNM分期、纵隔淋巴结转移有关，CXCL12、CXCR4专一性结合可能有促进淋巴结转移作用。 Objective By detecting the expression of CXC chemokine ligand 12 （CXCL12） and CXC chemokine receptor 4 （CXCR4） in non- small cell lung cancer （NSCLC） tissues and adjacent nor- real lung tissues, we try to investigate the correlation between the expression of CXCL12/CXCR4 and clinical parameters. Methods Immunohistochemical staining method was used to detect the expression of CXCL12 and CXCR4 in cancer tissues and matched normal lung tissues adjacent to carcinoma of 62 cases of NSCLC. Under the microscope at high magnification, positive staining （＋） was recorded when the number of positive cells accounted for more than or equal to 10%, and negative staining （ - ） was recorded when the number of positive cells accounted for less than 10%. The positive expression rate and the rela- tionship between the various clinical parameters were analyzed. Results The positive expression rate of CXCL12 in NSCLC tissues and normal lung tissue adjacent to carcinoma was 73.8% and 15.0% respec- tively （P = 0. 040）. The positive expression of CXCR4 was detected in 33 cases and 2 cases of NSCLC tis- sues and normal lung tissue adjacent to carcinoma respectively （P = 0. 030）. The positive expression rate of CXCL12 in stage Ⅲa - Ⅲb was 90. 9% , and 55.5% in stage Ⅱa - Ⅱb. The positive expression rate of CXCR4 in Ⅲa - Ⅲb was 90. 9% , and 55.5% in stage Ⅱa - Ⅱb （P =0. 030）. The positive expression rate of CXCL12 in mediastinal lymph node with metastasis was 94. 7% , and that in the mediastinal lymph node without metastasis was 56. 5%. The expression of CXCL12 and CXCR4 in NSCLC tissues was consistent. Conclusion CXCL12 and CXCR4 expression in NSCLC tissues has the effect to promote cancer. The positive expression of CXCL12 and CXCR4 is associated with TNM staging and mediastinal lymph node metastasis. The specific binding of CXCL12 and CXCR4 may promote lymph node metastasis.