摘要
多药外排泵由一系列转运蛋白组成,可以清除菌体内的抗生素,使细菌呈现固有耐药或获得性耐药,几乎所有的病原微生物都有这种耐药机制。已发现多种外排泵及其相关蛋白质,诱发外排泵的作用机制仍在研究,其中具有特异结合药物的外排泵相关蛋白已经用于抗生素残留检测的实践。笔者将重点叙述细菌的抗生素外排泵类型及其组成和调控蛋白的研究进展,以及外排泵蛋白在抗生素残留检测中的应用,并总结在外排泵研究方面存在的问题和发展趋势。
Multidrug efflux pumps are found in any organism and constitute a series of transport- ers, which contribute to the resistant to antibiotics intrinsic and acquired. Nowadays, numerous efflux pump-related proteins have been investigated, and the exact mechanism of induction drug extrusion is researching. Also, there are few efflux-related protein used in antibiotic residue de- tection. The aim of this review is to elucidate the types of efflux pumps and their constitutive and regulator proteins, and also the application of efflux pump proteins in antibiotic residue detection. Finally, the problems and developmental trends in the research of efflux pump are summarized and discussed.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2017年第11期2023-2033,共11页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家重点研发计划(2016YFD0501302
2017YFD0501406)
国家自然科学基金(31772791
31502115)
中央高校基础研究基金(2662015PY035)
国家畜禽产品质量安全风险评估(GJFP2016008)
华中农业大学博士科研创新项目(2014bs14)
关键词
抗生素
外排泵
组成蛋白
调节蛋白
残留检测
antibiotics
efflux pump
constitutive protein
regulator protein
residue detection
