摘要
目的探讨EBV感染引发IM与CAEBV患儿的外周血淋巴细胞亚群及免疫功能之间的差异,为临床诊断提供可靠的数据支持。方法回顾分析郑州市儿童医院儿科40例EBV患儿临床资料,IM患儿21例,CAEBV患儿19例;选择同期健康儿童20例。采用流式细胞仪检测外周血血常规及淋巴细胞亚群;使用比浊法检测血清免疫球蛋白水平。观察比较3组外周血血常规及淋巴细胞亚群检测结果、免疫球蛋白检测结果。结果三组外周血血常规及淋巴细胞亚群检测结果差异有统计学意义(P<0.05);其中,IM组患儿的外周血血常规检测结果及淋巴细胞亚群检测中的CD^+3T、CD^+4T、CD^+8T计数高于CAEBV组、正常组(P<0.05);CAEBV组B淋巴细胞、自然杀伤细胞计数低于IM组、正常组(P<0.05)。IM组与CAEBV组IgA、IgG水平高于正常组(P<0.05);但血清IgM水平三组比较差异无统计学意义(P>0.05)。结论 CD^+8T淋巴细胞计数差异是区分IM与CAEBV主要因素。检测外周血淋巴细胞亚群水平有利于临床对IM、CAEBV鉴别与诊治。
Objective To investigate the difference between peripheral blood lymphocyte subsets and immune function in children with IM and CAEBV caused by EBV infection,so as to provide reliable data support for clinical diagnosis.Methods The clinical data of 40 cases of children with EBV in our hospital was retrospectively analyzed,including 21 children with IM and 19 children with CAEBV. 20 healthy children were selected at the same time. Peripheral blood routine examination and lymphocyte subsets were detected by flow cytometry,and the serum immunoglobulin level was inspected by turbidimetry. The peripheral blood routine test,lymphocyte subsets test and immunoglobulin test outcomes were observed and compared among the three groups.Results The testing outcomes of three groups for peripheral blood routine examination and lymphocyte subsets showed difference（ P〈0. 05）; The CD~＋3 T,CD~＋4 T,CD~＋8 T counts in the peripheral blood routine examination and lymphocyte subsets test of IM group were higher than those of CAEBV group and normal group（ P〈0. 05）; B lymphocyte and natural killer cell count in CAEBV group were lower than those in IM group and normal group（ P〈0. 05）. The IM group and CAEBV group＇s IgA and IgG level were higher than those of normal group（ P〈0. 05）,but there was no difference in serum IgM level among the three groups（ P〈0. 05）. Conclusion The difference of CD~＋8 T lymphocyte counts is the major factor to distinguish IM and CAEBV. The detection of peripheral blood lymphocyte subsets level is beneficial to the differential diagnosis and treatment of IM and CAEBV.
出处
《医药论坛杂志》
2017年第10期61-63,共3页
Journal of Medical Forum
