摘要
目的:从南海鸢乌贼墨汁中分离纯化多糖,并分析其多糖组分。方法:利用水提醇沉法得粗多糖,通过固相萃取层析法除色素对多糖进行纯化,纯化多糖依次经DEAE-52离子交换柱、Sephacryl HR-300凝胶柱分级纯化,按峰收集得单一组分,采用1-苯基-3-甲基-5-吡唑啉酮(1-phenyl-3-methyl-5-pyrazolone,PMP)衍生化方法,通过超高效液相色谱分析多糖组分。结果:粗多糖经DEAE-52离子交换柱、Sephacryl HR-300凝胶柱后收集得到3个糖组分,分别为SIP1、SIP2、SIP3;通过PMP衍生化方法分析单糖组成,得:SIP1是由D-甘露糖、D-葡萄糖、D-半乳糖3种单糖缩合而成;SIP2和SIP3分别是由D-甘露糖、鼠李糖、D-葡萄糖、D-半乳糖及L-(-)-岩藻糖5种单糖按照不同比例缩合而成。
Objective：To isolate and purify polysaccharides from the ink of Sthenoteuthis oualaniensis in the South China Sea,and to analyze the monosaccharide composition of the purified polysaccharides.Methods：Crude polysaccharides were isolated through water extraction followed by alcohol precipitation and passed through a solid phase extraction column to remove the pigment.The polysaccharides were fractionated and further purified into homogeneity by sequential chromatographies on DEAE-52 ion exchange and Sephacryl HR-300 columns.The monosaccharide composition was analyzed by ultra performance liquid chromatography（UPLC） after 1-phenyl-3-methyl-5-pyrazolone（PMP） derivatization.Results：Three purified polysaccharides designated as SIP1,SIP2,and SIP3 were obtained.SIP1 was composed of D-mannose,D-glucose,and D-galactose while SIP2 and SIP3 were composed of D-mannose,rat sugar,D-glucose,D-galactose and L-（-）-fucose in different ratios.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2017年第24期118-123,共6页
Food Science
基金
农业部财政重大专项(NFZX2013)
国家重点基础研究发展计划(973计划)项目(2014CB441500)
关键词
墨多糖
分离纯化
组分分析
sepia ink polysaccharides
separation and purification
monosaccharide analysis
