摘要
目的:运用转录组学探究新琼四糖对过度疲劳性肠道损伤的保护作用。方法:雄性BALB/c小鼠被随机分为正常组、力竭运动组、阳性对照低聚果糖组和新琼四糖干预组,连续喂养16 d后,取小鼠结肠组织,应用RNA-seq技术对结肠组织RNA进行完全测序,并对测序结果进行分析。结果:过度疲劳引起小鼠结肠肿瘤标志物Zfp521和Zmynd8等锌指蛋白表达量极显著增加(P<0.01)、抑癌因子Casz1、Zfp346等的表达显著降低(P<0.05),新琼四糖可缓解过度疲劳造成的肠道损伤,下调肿瘤相关锌指蛋白的表达,上调抑癌锌指蛋白的表达。结论:新琼四糖可保护肠道、缓解过度疲劳引发的肠道损伤、预防肿瘤的发生。
Purpose： To explore the protective effect of neoagarotetraose against over-exhaustion-induced gut injury by RNA-seq analysis. Methods： Male BALB/c mice were randomly divided into normal control group, over-exhaustion model group （both the groups were gavaged with saline）, over-exhaustion plus fructooligosaccharide （FOS） group （positive control）, and over-exhaustion plus neoagarotetraose （NAT） group. After sixteen days of administration, colon tissues from the mice in each group were collected, and then RNA was extracted for RNA-seq analysis. Results： Over-exhaustion resulted in significantly increased expression of zinc finger proteins Zfp521 and Zmynd8, associated with inflammation and tumor proliferation （P 〈 0.01）, and significantly reduced expression of tumor suppressors Casz1 and Zfp346 （P 〈 0.05）. NAT could relieve over-exhaustion-induced gut injury by downregulating the expression of zinc finger proteins related to tumor proliferation and upregulating the expression of tumor-suppressing zinc finger proteins. Conclusion： NAT can protect against over-exhaustion-induced gut injury in mice and prevent the occurrence of tumors.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2017年第23期135-140,共6页
Food Science
基金
国家自然科学基金面上项目(31271923)
国家自然科学基金联合基金项目(U1406402)
