摘要
为研究EF-1a基因在毛壳菌(Chaetomium Kunze)属内种间的鉴别能力,采用23株毛壳菌进行EF-1a基因序列分析。通过碱基变异结果分析可发现23株毛壳菌的EF-1a基因体现出较大的变异性,通过邻近法构建EF-1a基因的系统发育树,23株毛壳菌被划分在8个分枝上,分别归属于7个不同的种。EF-1a基因不仅可以用来区分毛壳菌属的一些形态差异较大的种,如粪生毛壳(C.funicola),大果毛壳(C.megalocarpum)、球毛壳(C.globosum)和马德拉斯毛壳(C.madrasense),还可以用来区分一些形态上非常相似的种,如:印度毛壳(C.indicum)、粪生毛壳(C.funicola)和(C.erectum)、球毛壳(C.globosum)和近缘毛壳(C.subaffine)类群。同时,对一些通过形态特征不能确定的种也可以进行初步鉴定。因此,EF-1a基因可用于毛壳菌属内不同种的鉴定。
In order to discover identification ability of EF-1a in the genus Chaetomium Kunze, 23 strains were studied by sequence analysis. The results showed that 23 strains of Chaetomium spp. indicated great variability in EF-1a gene. Phylogenetic tree of EF-1a gene was constructed by neighbor-joining method. 23 strains were divided into 8 branches and belonged to seven different species. EF-1a gene can be used not only to distinguish some species with different morphological differences, such as C. funicola, C. megalocarpum, C. globosum, and C. madrasense, but also to distinguish between very similar morphological species, such as C. indicum, C. funicola and C. erectum, C. globosum and C. subaffine. Also, EF-1a gene can be used for identification of some species which could not be identified by morphological characteristic. EF-1a gene was suitable for identification of species within Chaetomium spp..
出处
《中国植保导刊》
北大核心
2017年第11期5-9,共5页
China Plant Protection
基金
国家自然科学基金(31360006)
作物学科建设项目(2015ZWXKJS
2016ZWXKJS)
