摘要
目的通过检测固定正畸患者不同矫治时期龈沟液(GCF)中P物质(SP)和白细胞介素-1β(IL-1β)含量的动态变化,探讨两者在正畸牙牙周组织改建过程中的影响和临床意义。方法选择2015年4月至2016年3月于泰安市中心医院需要拔除双侧第一前磨牙固定矫治患者30例,随机选择一侧尖牙作为观察组,施加250 g远中移动初始力,另一侧为对照组不受力。分别在加力当天以及加力后1 d、7 d、14 d、21 d和28 d收集患者尖牙近中及远中龈沟液,采用酶联免疫吸附实验(ELISA)测定SP和IL-1β含量。结果正畸加力后观察组GCF中SP和IL-1β均开始升高[(3.8±1.2)pg/m L、(42.4±11.5)ng/m L],第7天均达到高峰[(5.0±1.4)pg/m L、(53.8±13.7)ng/m L],第28天基本回落至基线[(2.6±0.8)pg/m L和(35.6±11.9)ng/m L]。对照组GCF中SP和IL-1β基本维持在基线。加力后1 d、7 d和14 d观察组GCF中SP含量和加力后1 d、7 d IL-1β含量与对照组比较差异有统计学意义(P<0.05)。结论正畸牙龈沟液中SP和IL-1β的表达随牙周组织受力变化而变化,参与牙齿移动和牙槽骨改建,其监测为正确判断正畸牙的牙周状况提供一定参考依据。
Objective To investigate the dynamic changes of substance P （SP） and interleukin-1β（IL-1β） lev-els in gingival crevicular fluid （GCF）, and to explore their influence and clinical significance on the construction of para-dentium during orthodontic toot movement. Methods The canines of 30 patients who admitted to Tai＆#39;an City Central Hospital from April 2015 to March 2016 and involving the extraction of the maxillary first premolar tooth were divid-ed randomly into two groups. The distal forces of 250 g were applied to the experimental canines and the other sides were not. The GCF was collected before activation and at 1 d, 7 d, 14 d, 21 d and 28 d respectively after the initiation of the experiment. The levels of SP and IL-1βin GCF were determined by enzyme-linked immunosorbent assay （ELI-SA） tests. Results The levels of SP and IL-1βin the experimental group began to increase at 1 d from （3.8±1.2） pg/mL and （42.4±11.5） ng/mL, respectively, and reached the peak of （5.0±1.4） pg/mL and （53.8±13.7） ng/mL at 7 d after the ex-periment, then returned to baseline of （2.6±0.8） pg/mL and （35.6±11.9） ng/mL at 28 d. Both of them in the control group maintained at baseline level during the experiment. The changes of the level of SP at 1 d, 7 d, 14 d and IL-1βat 1 d, 7 d were found statistically significant between the experimental and control groups （P〈0.05）. Conclusion The levels of SP and IL-1βin GCF is correlated with orthodontic force, which may be involved in tooth movement and alveolar bone remodeling in response to mechanical stress, and therefore they can be used as a diagnostic efficient method for monitor-ing periodontal health in clinical practice.
出处
《海南医学》
CAS
2017年第20期3331-3333,共3页
Hainan Medical Journal
