摘要
目的检测基质金属蛋白酶-11(MMP-11)和转录因子Sp1在结肠癌中的表达,探讨二者表达的相关性及临床意义。方法采用免疫组织化学方法检测72例结肠癌患者肿瘤组织及20例癌旁正常组织中MMP-11和转录因子Sp1的表达。结果 MMP-11和转录因子Sp1在72例结肠癌中表达的阳性率分别为63.89%(46/72)和66.67%(48/72),与在20例正常结肠组织中表达阳性率(分别为20%和10%)的差异有统计学意义(P<0.05)。MMP-11的过表达与结肠癌的TNM分期、分化程度及淋巴结转移相关(P<0.05),而与患者的年龄、性别、肿瘤大小无明显相关性;在结肠癌组织中MMP-11和转录因子Sp1的表达之间呈明显正相关(r=0.45,P<0.01)。结论 MMP-11和转录因子Sp1与结肠癌的生物学行为密切相关,可作为预测结肠癌浸润和转移潜能及评估预后的参考指标之一。
Objective To determine the expressions of matrix metalloproteinase-11（MMP-11） and transcription factor Sp1 in colorectal carcinoma,and to investigate their relations. Methods Immunohistochemical technique was used to determine the expressions of MMP-11 and transcription factor Sp1 in 72 specimens of colorectal cancer tissues and 20 specimens of adjacent normal colorectal tissues. Results The positive expression rates of MMP- 11 and transcription factor Sp1 in colorectal cancer tissues were 63.89%（46/72） and 66.67%（48/72）,and those in adjacent normal colorectal tissues were 20% and 10%（P〈0.05）. The expressions of MMP-11 in colorectal cancer tissues were correlated with TNM stage,differentiation degree and lymph node metastasis（P〈0.05）,but there was no correlation with age,sex and tumor size. MMP-11 expression was positively correlated with transcription factor Sp1 expression in colorectal cancer tissues（r=0.45,P〈0.01）. Conclusions MMP-11 and transcription factor Sp1 have close relations with the biological behavior of colorectal carcinoma,and it may be markers in predicting the invasion,metastasis and prognosis of colorectal carcinoma.
出处
《检验医学》
CAS
2017年第11期1017-1020,共4页
Laboratory Medicine
基金
河北省医学科学研究重点课题计划(20160365)
关键词
基质金属蛋白酶-11
转录因子SP1
结肠癌
预后
Matrix metalloproteinase-11
Transcription factor Sp1
Colorectal carcinoma
Prognosis
