摘要
为明确马缨丹叶片中分离提纯的马缨丹酸、马缨丹烯A和马缨丹烯B 3种三萜类化合物对白蚁的毒杀活性及对其体内纤维素酶的影响,采用滤纸片饲喂法测定了3种化合物对黄胸散白蚁的毒杀作用,并用酶标仪测定了不同浓度、不同处理时间白蚁体内内切葡聚糖酶(EG)、外切葡聚糖酶(CBH)、β-葡萄糖苷酶(BGL)活性。结果表明,3种三萜类化合物对白蚁具有一定的毒杀作用。酶活性测定表明,不同浓度马缨丹酸处理黄胸散白蚁12~72 h,白蚁体内的EG、CBH、BGL活性均有不同程度的变化,其对白蚁体内EG活性总体有抑制作用,2 mg/m L在12~36 h抑制作用最为显著;对CBH活性则有一定的促进作用,2 mg/m L对CBH活性促进作用较为明显,但与对照相比差异不显著;对BGL活性则表现为抑制作用。马缨丹烯B和马缨丹烯A对黄胸散白蚁体内3种酶活性均表现为抑制作用,且随浓度的增大抑制作用显著。因此,3种三萜类化合物对黄胸散白蚁体内的纤维素酶活性均有一定影响,尤其是马缨丹烯B、A抑制作用较强,它们对3种酶活性的影响与浓度和时间有关。
In order to define the bioactivity and effects on the cellulose activity of termite,three triterpenoids,lantanolic acid,lantadene A and B,which isolated and purified from leaves of Lantana camara,were used to feed Reticulitermes flaviceps by filter paper method. The activities of 3 enzymes of endo-1,4-β-glucanase( EG),exo-β-1,4-glucanase( CBH) and β-1,4-glucosidase( BGL) in termite were tested with ELIASA at different concentrations and treatment times. The results showed that the 3 triterpenoids all had certain toxic activity on termite. The enzyme activity test showed that EG,CBH and BGL all had different changes when treated with lantanolic acid for 12 ~ 72 hours. In general,lantanolic acid had an inhibitory effect on EG,and it was significant at the concentration of 2 mg/m L in 12 ~ 36 hours. Lantanolic acid had certain active effect on CBH,and the effect of 2 mg/m L was more obvious,but it was not significantly different compared with control. On BGL,lantanolic acid showed an inhibitory effect. Lantadene A and B showedinhibitory effects on all three kinds of enzymes,and the inhibitory effects became significant with the increase of concentration. Therefore,three triterpenoids all had certain effects on the cellulase activity in Reticulitermes flaviceps which depended on the concentration and treatment time,especially the inhibitory effect of lantadene B and A.
出处
《山东农业科学》
2017年第11期98-104,共7页
Shandong Agricultural Sciences
基金
国家自然科学基金项目(31272106)
山东省"泰山学者"建设工程专项
