摘要
目的探讨抗-HCV-IgG抗体与血清HCV RNA应用于丙型肝炎诊断中的临床价值。方法选择2014年10月~2016年10月检验科收集的114例丙型肝炎待查者的血清标本,采用酶联免疫吸附法(ELISA)对抗-HCV-IgG抗体进行检测,同时采用荧光定量聚合酶链反应(PCR)对HCV RNA载量进行检测。统计所有标本抗-HCV-IgG抗体、HCV RNA检测结果。结果采用ELISA检测抗-HCV-IgG抗体阳性率为25.44%(29/114);采用荧光定量PCR检测HCV RNA阳性率为27.19%(31/114)。31例HCV RNA阳性标本中抗-HCV-IgG阳性标本有26例,符合率为83.87%(26/31)。ELISA检测抗-HCV-IgG抗体阳性率与荧光定量PCR检测HCV RNA阳性率的差异无统计学意义(P>0.05)。伴随HCV RNA病毒载量的不断增多,抗-HCV-IgG阳性抗体检出率明显提高;不同HCV RNA病毒载量的相邻区间的抗-HCV-IgG阳性抗体检出率比较,差异无统计学意义(P>0.05)。结论 ELISA检测抗-HCV-IgG抗体和荧光定量PCR检测HCV RNA应用于丙型肝炎诊断均具有一定的临床价值,血清HCV RNA病毒载量是判断HCV感染的重要依据,能够有效反映病毒活动性及复制程度,然而单一的抗-HCV-IgG抗体、HCV RNA检测尚存在局限性,易出现漏诊,两者联合检测可显著提高丙型肝炎检出率,做到早诊断、早治疗,提高临床疗效。
Objective To investigate the clinical value of anti-HCV-lgG antibody and serum HCV-RNA in the diagnosis of hepatitis C. Methods The serum samples of 114 patients with hepatitis C were collected from October 2014 to October 2016, the anti-HCV-RNA antibody was detected by enzyme-linked immunosorbent assay (ELISA) , and the HCV-RNA load was detected by fluorescence quantitative polymerase chain reaction (PCR) .Statistics of all specimens of anti-HCV-RNA antibody, HCV-RNA data. Results The positive rate of anti-HCV-IgG was 25.44% (29/114) by ELISA. The positive rate of HCV-RNAwas 27.19% (31/114) by fluorescence quantitative PCR. anti-HCV-IgGpositive specimens of 26 cases were detected from 31 cases of HCV-RNA positive specimens, the coincidence rate of 83.87% (26/31) . There was no significant difference in detection positive rate between anti-HCV-IgG antibody by ELISA and HCV-RNA by fluorescence quantitative PCR (P〉 0.05) . With the increase of HCV-RNA viral load, the detection rate ofanti-HCV- IgG positive antibody was significantly increased. There was no significant difference in the detection rate of anti-HCV-lgG antibody positive in the adjacent region of different HCV-RNA viral load (P〉 0.05) .Conclusion ELISA to detect anti- HCV-RNA antibody and fluorescence quantitative PCR detection of HCV-RNA in the diagnosis of hepatitis C have a certain clinical value, serum HCV-RNA viral load is an important basis for judging the infection of hepatitis C virus, which can But a single anti-HCV-RNA antibody, HCV-RNA detection is still limited, prone to misdiagnosis phenomenon, the combination of the two can significantly improve the detection rate of hepatitis C virus infection, Do early diagnosis, early treatment, to provide value for improving clinical efficacy.
出处
《临床输血与检验》
CAS
2017年第6期602-605,共4页
Journal of Clinical Transfusion and Laboratory Medicine
基金
无锡市医院管理中心科研项目(No.YGZXH14010)资助