摘要
目的:探讨唑来膦酸对牙龈成纤维细胞增殖、凋亡、分化的影响及机制。方法:从牙龈组织分离原代人牙龈成纤维细胞(HGFs),0.5、1、5、10μmol/L的唑来膦酸处理HGFs 24、48、72h后,CCK8实验检测细胞的增殖;流式细胞仪检测10μmol/L的唑来膦酸处理HGFs 72h后的细胞凋亡情况;加入10μmol/L的唑来膦酸的成骨诱导分化液培养HGFs 7d后,碱性磷酸酶(ALP)试剂盒检测ALP活性,Western blot检测Runx2和OPN的蛋白表达;10μmol/L的唑来膦酸处理HGFs 72h后,Western blot检测NF-κB信号通路p65、IκBα蛋白表达。结果:唑来膦酸可呈剂量依赖抑制HGFs细胞增殖(P<0.01);唑来膦酸组细胞的凋亡率显著高于对照组,ALP活性及Runx2、OPN蛋白表达显著高于对照组,p65、IκBα的蛋白表达显著低于对照组(P<0.01)。结论:唑来膦酸可抑制HGFs增殖,促进细胞的凋亡和分化,其机制与下调NF-κB信号通路有关。
Objective：To investigate the effect and mechanism of zoledronic acid on proliferation,apoptosis,and differentiation of gingival fibroblasts.Methods：Primary human gingival fibroblasts（HGFs）isolated from the gingival tissue were treated with 0.5,1,5,and 10μmol/L zoledronic acid for 24,48,and 72 h.Cell proliferation was detected by CCK8 assay.Cell apoptosis was detected by flow cytometry in 10μmol/L zoledronic acid treated HGFs cells for 72 h.Alkaline phosphatase（ALP）activity was detected by ALP kit,the expression of Runx2 and OPN protein were detected by Western blot as well as p65 and IκBαprotein after treated with 10μmol/L zoledronic acid for72 h.Results：Zoledronic acid inhibited proliferation of HGFs in a dose dependent manner（P0.01）.Apoptosis rate of cells in zoledronic acid was significantly higher than that of control group.ALP activity and the expression of Runx2 and OPN protein were significantly higher than those of control group,whereas the protein expressions of p65 and IκBαwere significantly lower than the control group（P0.01）.Conclusion：Zoledronic acid can inhibit the proliferation but promote the apoptosis and differentiation of HGFs by downregulating NF-κB signaling pathway.
出处
《口腔医学研究》
CAS
北大核心
2017年第11期1169-1172,共4页
Journal of Oral Science Research
基金
黑龙江省自然科学基金项目(编号:H201596)
