摘要
目的:对水翁花中的熊果苷和2',4'-二羟基-6'-甲氧基-3'-5'-二甲基查耳酮进行含量测定研究,建立药材的质量标准。方法:采用高效液相色谱(HPLC)法:以Diamonsil-C_(18)(5μm,250×4.6 mm)为色谱柱,以乙腈-0.05%磷酸(60:40)为流动相进行梯度洗脱,检测波长254 nm,流速1.0 mL/min,柱温25℃;结果:采用HPLC法建立了8批药材的指标性成分指纹图谱并进行准确定量;结论:该方法快速、高效、准确,能够全面、有针对性地对水翁花药材的质量优劣进行评价。
Objective： To evaluate the quality of Flos Cleistocalycis Operculati by quantitatively determining arbutin and 2＇,4＇-dihydroxy-6＇-methoxy-3＇-5＇-dimethylchalcone. Methods： HPLC was used for determination,with Diamonsil-C（18）（ 5 μm,250 × 4. 6 mm） column and a mobile phase containing acetonitrile-0. 05% phosphoric acid; the detection wavelength was 254 nm,column temperature 25 ℃ and the elution mode was gradient,with a flow rate of 1. 0 mL/min; Results： Eight batches of herb samples were accurately determined and established fingerprints. Conclusion： The proposed method was fast,efficient and accurate,which can be used in quality evaluation of Flos Cleistocalycis Operculati comprehensively and specifically.
出处
《世界中医药》
CAS
2017年第10期2474-2477,共4页
World Chinese Medicine
基金
广西中医药研究院系统研究课题(桂中重自201401)
关键词
水翁花
高效液相色谱法
活性成分
Flos Cleistocalycis Operculati
High performance liquid chromatography
Active components
