摘要
目的从形态学角度探讨二补助育汤对胚胎着床障碍模型小鼠子宫内膜的影响。方法将56只C57小鼠随机分为空白组、模型组、补佳乐组、阿司匹林组、二补助育汤低、中、高剂量组,每组8只。用吲哚美辛建立胚胎着床障碍动物模型,小鼠妊娠第5日下午脱颈椎处死,计算各组小鼠妊娠率及胚胎着床位点数;光学显微镜下观察各组小鼠子宫内膜形态结构;透射电子显微镜下观察超微结构、胞饮突表达情况,并计算胞饮突评分。结果 (1)模型组平均着床位点数(1.8±2.1)明显低于空白组(8.1±4.2)(P<0.05);中药中、高剂量组平均着床位点数(分别为62.5%,75.0%)明显高于模型组(P<0.05);与补佳乐组和阿司匹林组间无统计学差异。(2)光学显微镜下形态学观察:正常组内膜间质疏松、腺体和血管丰富、腺体扩张;模型组内膜变薄或消失、腺体和血管减少;二补助育汤各剂量组、补佳乐组、阿司匹林组内膜均有改善,以二补助育汤中剂量组与正常组最接近。(3)电子显微镜下超微结构观察:空白组子宫内膜上皮细胞及细胞器结构清晰;模型组细胞破坏严重甚至局灶性坏死,线粒体膜不完整、嵴结构消失,胞质中较多空泡样变,核糖体和内质网明显减少;二补助育汤各剂量组、补佳乐组、阿司匹林组细胞及细胞器有不同程度破坏,与模型组相比有明显改善。(4)胞饮突:空白组胞饮突丰富,绒毛较少;模型组绒毛丰富,但胞饮突很少;二补助育汤各剂量组胞饮突较模型组丰富,见少量绒毛;补佳乐组和阿司匹林组可见少量胞饮突,较模型组增加,绒毛表达稀疏。(5)胞饮突评分:模型组胞饮突评分(1.1±0.3)较空白组低(2.5±0.7)(P<0.05);中药中剂量组(2.3±0.9)、阿司匹林组(2.0±0.7)评分高于模型组(P<0.05),亦高于补佳乐组(1.3±0.5)(P<0.05)。结论二补助育汤能够促进子宫内膜发育及胞饮突,从而提高子宫内膜容受性,有利于胚胎着床。
Objective To explore the effects of Erbu Zhuyu decoction on endometrial morphology in mouse model of embryo implantation dysfunction. Methods A total of 56 C57 mice were randomly divided into seven groups：blank group, model group, progynova group, aspirin group, and low-dose group, middle-dose group, high-dose group of Erbu Zhuyu decoction before built into embryo implantation dysfunction model by indometacin, 8 mice in each group. Indomethacin was used to build the mouse of embyo implantation dysfunction. The mice were killed at day5 of pregnancy, and the pregnancy rate and the number of embyo implantation sites were calculated. Endometrial morphology was observed by light microscope, ultrastructure of endometrial cells and pinopodes was observed by transmission electron microscope（TEM）, and pinopodes expression was scored. Results The number of implantation sites in model group（1.8±2.1） was less than that in blank group（8.1±4.2）（P0.05）, which was more in middle-dose group（7.0±6.2） and high-dose group（7.9±5.2） than that in model group（P0.05）, but were not significantly different from western medicine groups（progynova group, aspirin group）. For morphologic study： in blankgroup, there were loose endometrial stroma, abundant glands and vessels, which were opposite in model group, and were better in other groups than in model group, especially in the middle-dose of Erbu Zhuyu decoction group. For ultrastructure study： endometrial epithelial cell structure and organelle structure were clear in blank group. The cells were severely damaged and even focally necrosed in model group, organelle structure was also damaged, such as integrated mitochondrial membrane and disappeared cristae structure, decreased ribosome. They were better in others groups than in model group. For pinopodes expression： in blank group, pinopodes expression was rich, while pinopodes expression was much less and more villi were expressed in model group. Pinopodes expression was abundant in Erbu Zhuyu decoction groups, more than model group. In western medicine groups, pinopodes expression was not as good as that in Erbu Zhuyu decoction groups, but better than model group. The pinopodes scores in model group（1.1±0.3） was lower than those in blank group（2.5±0.7）（P0.05）, which were higher in middle-dose group（2.3±0.9）and asprin group（2.0±0.7） than that in model group and progynova group（1.3±0.5）（P0.05）. Conclusion Erbu Zhuyu decoction could improve endometrial receptivity by the way of regulating endometrial development and pinopodes expression.
出处
《中华生殖与避孕杂志》
CAS
CSCD
北大核心
2017年第10期808-814,共7页
Chinese Journal of Reproduction and Contraception
基金
国家自然科学基金(81473721)
2017年北京中医药大学在读研究生自主课题(2017-JYB-XS-098)~~
关键词
二补助育汤
胚泡着床障碍
子宫内膜容受性
形态学
胞饮突
Erbu Zhuyu Decoction
Model of embryo implantation dysfunction
Endometrial receptivity
Morphology
Pinopode
