摘要
目的探讨细胞毒性T淋巴细胞相关抗原4(CTLA-4)基因第一外显子+49A/G基因多态性与1型糖尿病(T1DM)的相关性。方法采用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RELP)分析技术分析30例T1DM患者及50例正常对照组的CTLA-4基因第一外显子+49A/G多态性,应用IBMSPSS Statistics 22统计软件进行分析处理,各组基因型和等位基因分布的比较采用χ2检验。结果 T1DM组等位基因+49A/G频率明显高于正常对照组(P<0.05),基因型GG型明显高于正常对照组,差异有统计学意义(P<0.05)。结论 CTLA-4基因第一外显子+49A/G多态性与T1DM的易感性相关,其中携带GG型基因型的父母其子女患T1DM的风险性增高。
Objective To study cytotoxic T lymphocyte associated antigen 4(CTLA 4) gene exon 1+49 A/G polymorphism with type 1 diabetes(T1 DM) relevance.Methods The polymerase chain reaction-estriction enzyme fragment length polymorphism(PCR-RELP) analysis technology of 50 cases of normal control group and of30 patients with T1 DM CTLA 4 gene exon 1+49 A/G polymorphism, application of the IBM SPSS Statistics 22 statistical software analysis processing,the comparison of each genotype and allele distribution using χ2 test.Results T1 DM group+49 A/G allele frequency was significantly higher than that of normal control group(P<0.05),genotype GG type was significantly higher than that of normal control group, the difference was statistically significant(P<0.05).Conclusion CTLA 4 gene exon 1+49 A/G polymorphism is associated with the susceptibility of T1 DM,among them with the GG genotype of parents children has increased the risk of T1 DM.
出处
《云南医药》
CAS
2017年第6期569-571,共3页
Medicine and Pharmacy of Yunnan
基金
昆明医科大学附属延安医院研究课题基金项目(项目编号:2013-038)
