摘要
目的通过测定120株不同来源肠球菌耐药性分布情况,毒力基因cylA、efaA、gelE、esp、ace和AS的携带率,旨在了解肠球菌耐药现状和毒力基因分布情况,为选择临床治疗方案和探索肠球菌致病机制提供参考。方法设计6对特异性引物,应用PCR方法对分离到的120株肠球菌进行6种毒力基因检测,同时用纸片扩散法对11种常用抗生素进行耐药性试验。结果 120个肠球菌分离株对抗生素均具有3重以上的耐药性,且不同来源菌株的多重耐药性有明显差异,其中以病死猪内脏分离株多重耐药性最为严重;总体上,上述分离株对林可霉素耐药率最高,为达96.00%(115/120),对环丙沙星、卡那霉素、四环素和达福普汀的耐药率均达到50%以上,而对替考拉宁的耐药率最低,仅为0.83%(1/120)。6种毒力基因cylA、efaA、gelE、esp、ace和AS在受试菌株中的阳性检出率依次为39.17%、56.67%、68.33%、23.33%、26.67%和13.33%;不同来源的肠球菌毒力基因携带率不同,且携带毒力基因数量也有明显差异,其中,来自病死猪内脏的肠球菌分离株携带毒力基因的比例较高,且有超过50%的菌株同时携带4种毒力基因及以上。结论肠球菌的耐药率和多重耐药性与毒力基因携带阳性率有一致性,且与菌株来源关系密切,因此对这一传统意义上的机会性病原体值得深入研究其中的因果关系。
One hundred and twenty enterococcal isolates,which were identified from animal specimen,were investigated for the drug-resistance and virulence gene status of Enterococci,so as to provide reference for clinical therapy as well as exploration of Enterococcus pathogenicity.For the 120 Enterococcusisolates,six virulence genes cylA,efaA,Gele,esp,ace and AS were detected with PCR method,and drug-resistance of 11 commonly used antibiotics were measured by K-B disk diffusion tests.The results showed that all of the 120 isolates presented resistance to at least 3 kinds of drugs,and multi-resistance occurred among different originated strains,especially prominent among those isolates from dead swine organs.Generally,the isolates performed highest resistance ratio against lincomycin(115/120),and more than 50 percent isolates were resisted to ciprofloxacin,kanamycin,tetracycline and dalfopristin,while the isolates were least resistant to teicoplanin(1/120).The incidence of cylA,efaA,GelE,esp,ace and ASvirulence genes,as a whole,was 39.17%,56.67%,68.33%,23.33%,26.67% and 13.33%,respectively,but it varied markedly among those of different sources,which showed consistent trend with that drug resistance.It is concluded that the drug-resistance incidence among the test enterococcal isolates showed correlation with that of virulence gene status,and both were closely related to the bacteria origin,therefore,the conventional opportunistic Enterococcus is of value for further exploring these profound relations.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2017年第11期984-990,共7页
Chinese Journal of Zoonoses
基金
河南省重点科技攻关项目(No.152102310081)~~
关键词
动物
肠球菌
毒力基因
耐药性
聚合酶链式反应
animal
Enterococcus
virulence gene
drug-resistance
polymerase chain reaction
