摘要
目的 :研究新型姜黄素对小鼠急性酒精性肝损伤的预防和保护作用。方法 :50只小鼠被随机均分为正常组、模型组和受试物组。正常组每天灌胃蒸馏水,模型组每天灌胃酒精,受试物组每天分别灌胃酒精和水飞蓟素、姜黄素的混合液。4周后,检测血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、甘油三酯(TG)含量,检测被测试动物血清肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)指标。肝组织行苏木精-伊红染色观察肝脏病理改变。结果 :与模型组相比,干预组能显著降低ALT、AST和TG的含量;血清TNF-α、IL-6的水平明显降低;改善肝细胞的脂肪性病理改变。结论 :姜黄素能减轻肝损伤,对小鼠酒精性脂肪肝具有良好的干预作用,其作用机制可能与抑制TNF-α和IL-6因子的表达有关。
Objective ：To study the preventive and protective effects of curcumin on mice with acute alcoholic liver injury. Methods ：50 mice were randomly divided into 5 groups,i.e. the normal group,model group,control group and curcumin low-dose group,curcumin high-dose group. All the animal were given intragastric administration of alcohol,silymarin and curcumin every day except the normal group mice. After 4 weeks,all the mice were taken blood from the eyes and killed,the contents of serum alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,and triglyceride（TG）were determined;the liver of TNF-α and IL-6 in serum by ELISA analysis. Results ：Compared with the model group,the intervention group can significantly reduce the contents of ALT,AST and TG;the levels of TNF-α and IL-6 decreased significantly in serum;the fatty pathological changes of liver cell were improved. Conclusion ：This study showed that curcumin had remarkable protective effect on mice with alcoholic liver injury,possibly due to its capability for inhibited the expression of TNF-α and IL-6.
出处
《辽宁中医药大学学报》
CAS
2017年第11期29-31,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
安徽中医药大学校级探索性科研资助项目(2017ts004)
安徽中医药大学科研资助项目(2015zr004)
