摘要
为探讨洋葱槲皮素的抗炎作用,本研究以小鼠腹腔巨噬细胞为模型,采用MTT法检测洋葱槲皮素单药组、洋葱槲皮素与LPS复合药组对巨噬细胞吞噬功能的影响;采用ELISA法检测洋葱槲皮素单药组、洋葱槲皮素与LPS复合药组对小鼠腹腔巨噬细胞分泌细胞因子和炎症介质的影响。结果显示,洋葱槲皮素能够提高巨噬细胞的吞噬功能;洋葱槲皮素单药、洋葱槲皮素与LPS复合药组刺激巨噬细胞,均能够抑制巨噬细胞IL-1β和COX-2的产生。结论:洋葱槲皮素可能通过提高巨噬细胞的吞噬功能、影响细胞因子和炎症介质的分泌,而发挥抗炎作用。
To discuss the inhibiting effect of onion quercetin on inflammatory response, this paper took mice＇ s enterocoelia macrophage as sample and used MTr to investigate the impact of onion quercetin single drug group, combination drug of onion quercetin and LPS group on macrophage s phagocytosis, used ELISA to investigate the impact of onion quercetin single drug group,combination drug of onion quercetin and LPS group on cytokines and inflammatory mediator secretion from mice＇ s enterocoelia macrophage.The result showed that onion quercetin can improve phagoeytosis of macrophage;onion quercetin single drug and combination drug of onion quercetin and LPS can stimulate macrophage of IL-1/3 and COX-2 which induces secretion of macrophage.In summary, onion quercetin may inhibit inflammation via improving macrophage＇ s phagocytosis and affecting secretion of cytokines and inflammatory mediator.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第23期284-288,共5页
Science and Technology of Food Industry
基金
辽宁省科技厅-锦州医科大学联合基金项目(20170540357)
辽宁省大学生创新创业训练计划项目(201610160000055
201610160000011)
关键词
洋葱槲皮素
巨噬细胞
细胞因子
炎症介质
抗炎
onion quercetin
macrophages
cytokines
inflammatory mediator
anti- inflammatory
