摘要
目的建立一种适用于同时测定康力欣胶囊中9种主要成分含量的HPLC波长切换联合梯度洗脱法。方法 Kromasil C18色谱柱(4.6 mm×250 mm,5μm);以乙腈-甲醇(1∶2,V/V)(A)-0.1%磷酸溶液(B)为流动相,梯度洗脱;流速0.9 ml/min;柱温30℃;检测波长分别为225 nm(木香烃内酯、去氢木香内酯)、254 nm(芦荟大黄素、大黄酸、大黄素、大黄素甲醚)和425 nm(双去甲氧基姜黄素、去甲氧基姜黄素、姜黄素)。结果这9种主要成分质量浓度依次分别在6.610~132.2(r=0.9999)、7.890~157.8(r=0.9996)、14.07~281.4(r=0.9992)、3.450~69.00(r=0.9997)、2.670~53.40(r=0.9998)、3.760~75.20(r=0.9999)、5.880~117.6(r=0.9996)、8.490~169.8(r=0.9993)和13.91~278.2μg/ml(r=0.9991)范围内线性关系良好,平均加样回收率依次分别为98.28%、97.76%、99.08%、97.19%、98.45%、96.96%、98.51%、97.52%和100.04%,RSD依次分别为1.09%、0.80%、1.72%、1.00%、1.24%、1.21%、1.55%、0.83%和0.93%。结论所建立的方法准确、快速,可用于康力欣胶囊的质量控制。
Objective To develop an HPLC wavelength switching combined gradient elution method for simultaneous determination of nine components in Kanglixin Jiaonang（KLXJN）. Methods The analysis was performed on a Kromasil C18（4.6 mm×250 mm,5 μm）with gradient elution by using the mobile phase of acetonitrile-methanol（1∶2）（A）-0.1% phosphoric acid solution（B）. The column temperature was maintained at 30℃ and the flow rate was 0.9 ml/min. The detection wavelengths were set at 225 nm for costunolide（1）and dehydrocostus lactone（2）,254 nm for aloe-emodin（3）,rhein（4）,emodin（5）and physcion（6）,and 425 nm for bisdemethoxycurcumin（7）,demethoxycurcumin（8）and curcumin（9）. Results The calibration curves were linear within the range（μg/ml）of 6.610-132.2（r=0.9999）for 1,7.890-157.8（r=0.9996）for 2,14.07-281.4（r=0.9992）for 3,3.450-69.00（r=0.9997）for 4,2.670-53.40（r=0.9998）for 5,3.760-75.20（r=0.9999）for 6,5.880-117.6（r=0.9996）for 7,8.490-169.8（r=0.9993）for 8,and13.91-278.2（r=0.9991）for 9,respectively. The recoveries for 1,2,3,4,5,6,7,8 and 9 were 98.28%（RSD=1.09%）,97.76%（RSD=0.80%）,99.08%（RSD=1.72%）,97.19%（RSD=1.00%）,98.45%（RSD=1.24%）,96.96%（RSD=1.21%）,98.51%（RSD=1.55%）,97.52%（RSD=0.83%）,and 100.04%（RSD=0.93%）,respectively. Conclusion The established method is accurate,rapid and can be used for the quality control of KLXJN.
出处
《国际药学研究杂志》
CSCD
北大核心
2017年第8期817-822,共6页
Journal of International Pharmaceutical Research
