摘要
采用^(60)Coγ射线源对青海弧菌Q67辐照,研究了辐射剂量(率)对其发光强度的影响。实验表明,在0.05 Gy·min^(-1)、0.1 Gy·min^(-1)、0.2 Gy·min^(-1)三种剂量率下,青海弧菌Q67经过短时间的照射后,其发光强度明显受到抑制。剂量率(d)和照射时间(t)两个变量中,d的增加对于青海弧菌Q67发光强度影响更大,并且通过软件SPSS19.0进行回归拟合分析,发现青海弧菌Q67的相对发光值(RLV)与d和t的自然对数值呈现二元线性关系。最后,以0.2 Gy·min^(-1)的剂量率梯度为对象,测定了辐照后青海弧菌Q67光密度(OD600)的变化,发现经过不同短时间辐照后相对光密度(ROD)也受到抑制,说明辐照引起的青海弧菌Q67菌体的损伤和死亡是其发光强度抑制的重要原因。研究表明,青海弧菌Q67对于γ辐照有较好的敏感性,可以作为一种潜在的评价γ射线对生命体危害的生物材料。
Background： The suppression of bacterial luminescence by poisonous substance has being previously reported, such as heavy metals, pesticides, however, rarely radiation. Purpose： This study aims to investigate the toxicity of the bioluminescent bacteria, Vibrio Qinghaiensis sp. Q67, to y-ray radiation and the possibility of using bacteria as a biological radiation dosemeter. Methods： In this paper, the effects of γ-ray radiation on luminous intensity of Vibrio Qinghaiensis sp. Q67 are studied using 60^Co γ radiation source with various dose rate （d） and irradiation time （t）. Results： Experimental results show that the relative luminous values （RLV） of Vibrio Qinghaiensis sp. Q67 decrease with the increase of dose rate （d） and irradiation time （0 for acute radiation, and linear bivariate associations is found between RLV and the logarithm value of d and t. Conclusion： Vibrio Qinghaiensis sp. Q67 is sensitive to acute y-ray radiation, which could be used to monitor ,γ-ray radiation.
出处
《核技术》
CAS
CSCD
北大核心
2017年第12期49-54,共6页
Nuclear Techniques
基金
国家自然科学基金(No.11575114)资助~~
