摘要
目的探讨贵州省3个少数民族线粒体DNA(mtDNA)9bp序列缺失频率情况。方法随机选取贵州省雷山县苗族(69例)、荔波县布依族(70例)、荔波县水族(44例)共183例男性血液标本,应用PCR及直接测序检测线粒体DNA 9bp序列缺失情况。结果在贵州省3个少数民族样本中发现标准型、缺失型、3型,缺失频率最高为荔波县水族(40.91%),雷山县苗族中检出3型1例,3个少数民族群体间mtDNA 9bp缺失频率比较差异无统计学意义(P>0.05)。结论 3个世居少数民族mtDNA 9bp缺失频率不同,水族的遗传变异较大,水族与苗族的亲缘关系相比水族与布依族较近。
Objective To study the frequency of 9 bp sequence deletion of mithochondrial DNA in three ethnic nationalities of Guizhou Province.Methods A total of 183 male blood samples were randomly extracted from Miao nationality（69 cases）in Leishan County,Shui nationality（44 cases）and Buyi nationality（70 cases）in Libo County.Polymerase chain reaction（PCR）and direct sequencing were used to detect the mithochondrial DNA 9 bp sequence deletion.Results The standard type,deleting type and3 type were found in the samples of 3 ethnic nationalities in Guizhou Province,the highest deletion frequency was Shui nationality（40.91%）in Libo County,1 cases of 3 type was detected in Miao nationality of Leishan County,and the mtDNA 9 bp deletion frequency had no statistical difference among 3 ethnic nationalities（P0.05）.Conclusion The frequency of mtDNA 9 bp deletion is different among three native minorities,the genetic variation in Shui nationality is larger,compared with Miao nationality genetic relationship,Shui nationality has a relatively closer affinity with Buyi nationality.
出处
《重庆医学》
CAS
北大核心
2017年第33期4702-4704,共3页
Chongqing medicine
基金
贵州省"2011协同创新中心"项目(黔教合协同创新中心[2014]06号)
贵州省科技厅基金资助项目(黔科合J字[2011]2119号)
关键词
贵州
布依族
水族
苗族
MTDNA
Guizhou
Buyi nationality
Shui nationality
Miao nationality
mtDNA
