摘要
采用响应面优化法对红曲产Monacolin K的固态发酵条件进行了优化,结果表明:最佳发酵条件为氮源(玉米粉)1%、碳源(淀粉)4%、初始pH 5.0、培养温度28℃、装米量31.77g/250mL三角瓶、初始含水量40.72%、接种量11.14%(V/V),该设计中模型预测的Monacolin K最大值为0.256%,进行重复验证实验,得到Monacolin K实测值为0.258%,与模型预测值接近。此结果可为大规模生产功能性红曲提供一定的理论基础。
In order to explore the effects on metabolic capacity of Monacolin K, use the response surface method to optimize the solid-state fermentation conditions for Monascus strains. The results show that the optimal fermentation conditions are obtained, nitrogen source (corn powder) 1%, carbon source (starch) 4%, initial pH of 5.0, cultivation temperature of 28 ℃, amount of rice of 31.77 g/250 mL triangular flask, initial moisture content of 40.72%, inoculum size of 11.14% (V/V). The maximum model prediction value of Monacolin K in the design is 0. 256%, the repeated validation experiments are carried out with the best experimental conditions in the model, and the measured value of Monacolin K is close to the predicted value of 0. 258% ; which can provide a certain theoretical basis for large-scale production of functional Monascus.
出处
《中国调味品》
北大核心
2017年第12期38-43,49,共7页
China Condiment
基金
四川省泸州市科技计划(2015CDLZ-S07)
关键词
红曲
Monacolin
K
固态发酵条件
响应面法
Monascus
Monacolin K
solid-state fermentation conditions
response surface method