摘要
目的:探讨一次大负荷运动后大鼠骨骼肌内质网应激的钙信号机制,并观察针刺干预对其的影响。方法:8周龄雄性SD大鼠128只随机分为空白对照组(C)、单纯运动组(E)、单纯针刺组(A)和运动针刺组(EA),其中E组和EA组进行一次大负荷运动,A组和EA组施以针刺干预。各组大鼠又按照运动后不同时间点分0 h、12 h、24 h、48 h和72 h组,在对应时相取比目鱼肌进行指标测试。使用TMEM法观测内质网钙离子浓度([Ca^(2+)]_(ER));ELISA法测定Ca^(2+)-ATP酶(SERCA)含量;应用Western blot法检测内质网应激蛋白GRP78、CRT表达。结果:1)一次大负荷运动后大鼠骨骼肌[Ca2+]ER急剧下降;SERCA含量亦明显下降;内质网应激蛋白GRP78、CRT表达明显上调,峰值出现在运动后即刻。2)针刺干预可使运动大鼠骨骼肌[Ca^(2+)]_(ER)和SERCA含量有所升高;GRP78和CRT蛋白表达相应下调。结论:一次大负荷运动可使骨骼肌内质网钙离子紊乱和相关酶功能障碍,进而诱导内质网应激的发生;运动后针刺可改善骨骼肌内质网功能受损,逐渐恢复[Ca^(2+)]_(ER)且有效调节内质网应激程度。
Objective： To investigate the influence of heavy load exercise on calcium signal of endoplasmic reticulum stress in skeletal muscle of rats,and to observe the regulative effect of acupuncture. Methods： One hundred and twenty-eight 8-week old male SD rats were randomly divided into control group（ C）,simple exercise group（ E）,simple acupuncture group（ A）,and exercise ＋ acupuncture group（ EA）. Group E and group EA carried out a heavy load downhill running,while group A and group EA were treated with acupuncture. The rats＇ soleus muscle was obtained at 0 h,12 h,24 h,48 h and 72 h after exercise and tested. The concentration of Ca^2＋（[Ca^2＋]ER） was determined by TMEM,the activity of Ca^2＋-ATPase（ SERCA） was tested by ELISA,and the expression of GRP78 and CRT were detected by Western blot. Results： 1） After heavy load exercise,[Ca^2＋]ER of skeletal muscle in rats decreased sharply and SERCA also decreased; GRP78 and CRT expressions increased,and their peak value appeared immediately after exercise; 2） acupuncture increased the activity of [Ca^2＋]ER and SERCA of skeletal muscle in exercised rats,and decreased GRP78 and CRT expressions. Conclusions： A heavy load exercise can cause Ca^2＋ disorders of endoplasmic reticulum and related enzyme dysfunction in skeletal muscle,and then induce endoplasmic reticulum stress; acupuncture can improve the impaired function of endoplasmic reticulum in skeletal muscle,gradually recover [Ca^2＋]ER and effectively regulate the level of endoplasmic reticulum stress.
出处
《北京体育大学学报》
CSSCI
北大核心
2017年第11期49-55,共7页
Journal of Beijing Sport University
基金
国家自然科学基金资助项目(编号:31471133)
成都体育学院青年基金资助项目(编号:15YJQN08)
关键词
运动
骨骼肌
内质网应激
钙离子
针刺
exercise
skeletal muscle
endoplasmic reticulum stress
calcium ion
acupuncture
