摘要
为了研究鸡Ⅱ型抗菌肽LEAP-2的体外表达产物的活性,试验进行了肝脏样品无菌采集,基因的克隆及密码子优化和表达,以表达产物对致病菌做纸片法药敏试验,判断其抑菌作用。结果表明,试验经低温处理获得了较好的肝脏总RNA,克隆并优化了鸡LEAP-2基因;经诱导得到了表达产物,形式为包涵体;抑菌试验表明,表达产物对16株分离致病菌具有抑制作用。
To determine the antibiotic activity of chicken LEAP-2 expressed in vitro, the gene of LEAP-2 was applied by RT-PCRinstructed by the sequence NM_001001606, and then was done with codon optimization and cloned into pGEX-6P-1. After expressed inE. coli DE3, the expressed product was managed to do antibiotic experiment by bacteriostasis circle test. The results showed that chickenLEAP-2 gene hada satisfied gain under liquid nitrogen processing, LEAP-2 was cloned and after optimized then expressed by incusionbodies type, antimicrobial susceptibility test result showed that 16 E. coli strain owned in laboratory was sensitived to the protein.
出处
《山西农业科学》
2017年第12期1998-2000,共3页
Journal of Shanxi Agricultural Sciences
基金
山西省科技攻关项目(20140311021-3)
关键词
鸡
LEAP-2
抗菌肽
原核表达
抑菌试验
chicken
LEAP-2
antimicrobial peptide
prokaryotic expression
bacteriostatic test
