摘要
研究旨在尝试利用体细胞克隆技术进行地方优良品种吕梁黑山羊的克隆保种。剪取吕梁黑山羊耳缘组织,通过组织块培养法获得了吕梁黑山羊皮肤成纤维细胞,使用第6代的皮肤成纤维细胞作为供体细胞进行核移植,将重构胚胎通过手术移植到受体辽宁白绒山羊母羊体内,结果成功获得了2只体细胞克隆黑山羊,生长发育状况良好,且与供体黑山羊一致,表明体细胞克隆技术是可行的。这是山西省首例吕梁黑山羊克隆个体,它的成功为优良地方品种改良、保种等研究提供了有效可行的方法,为下一步进行吕梁黑山羊的转基因育种研究奠定了技术基础。
The objective of this study was to cloneL俟liang black goat using the SCNT （somatic cell nuclear transfer）technologyand to protect local variety.A piece of ear tissue was collected fromL俟liang black goat and cultured to get skin fibroblast cells. Thereconstructed embryos were produced by nuclear transfer using the G6 generation ofL俟liang black goat ear firbroblast cells as donorsand Liaoning white cashmere goat ooctyes as receptors, and we successfully cloned twol俟liang black lambs on June 24, 2017 which wereconsistent with the donor. The result indicated thatL俟liang black goat was successfully cloned by SCNT technology. This study obtainsthe first cloned offspring ofL俟liang black goat, which will providea effective feasible method for breeding, and laya foundation for nextstep that produce transgenetic goats by somatic nuclear transfer.
出处
《山西农业科学》
2017年第12期2001-2005,共5页
Journal of Shanxi Agricultural Sciences
基金
山西省农业科学院科技自主创新能力提升工程项目(2017ZZCX-02)
关键词
吕梁黑山羊
克隆
保种
核移植
L俟liang black goat
clone
breed conservation
somatic cell nuclear transfer
