摘要
以11-巯基十一烷酸(11-MUA)为还原剂和保护剂,通过一步水热法合成了具有强烈荧光的水溶性金纳米簇(AuNCs),基于Cu^(2+)修饰的AuNCs@11-MUA构建了"关-开"型荧光探针用于多巴胺(DA)的选择性、高灵敏检测.向AuNCs@11-MUA溶液中加入Cu^(2+)离子后,AuNCs@11-MUA的荧光发生猝灭,体系的荧光信号处于"关闭"状态.在DA存在下,由于DA与Cu^(2+)具有更强的结合力,形成比Cu^(2+)/AuNCs@11-MUA复合体更稳定的络合物,可将Cu^(2+)从AuNCs@11-MUA表面移除下来,从而使其荧光得以恢复,体系的荧光信号呈"打开"状态.AuNCs@11-MUA探针的荧光恢复程度与DA的浓度在2.0×10^(-7)~5.0×10^(-5)mol/L范围呈良好的线性关系,检出限为8.0×10^(-8)mol/L(S/N=3).将该探针应用于人血清和尿液中DA的检测,回收率为93.2%~97.3%,相对标准偏差RSD<4.08%,表明该方法可应用于人体内多巴胺的检测.
One-step hydrothermal method was used for the preparation of water-soluble gold nanoclusters（Au NCs） with strong fluorescence using 11-mercaptoundecanoic acid（11-MUA） as reducing agent and protective agent.A turn-on fluorescent probe based on Cu^（2＋） modified AuNCs@11-MUA was developed for the detection of DA with high sensitivity and selectivity.After Cu^（2＋）ions were added into AuNCs@11-MUA solution,the fluorescence of AuNCs@11-MUA was quenched,and the fluorescence signal of the system was in the ＂off＂ state.In the presence of DA,a more stable complex than the AuNCs@11-MUA-Cu^（2＋）complex was formed due to DA and Cu^（2＋） have a stronger binding force,which resulted in the departure of Cu^（2＋） from the surface of the AuNCs@11-MUA,and the fluorescence signal of the system was ＂open＂.The fluorescence recovery degree of the AuNCs@11-MUA probe showed a good linear relationship with the concentration of DA from 2.0×10^（-7） mol/L to 5.0×10^（-5） mol/L.The limit of detection was estimated to be 8.0×10^（-8） mol/L（S/N=3）.The present method was applied for the detection of DA in human serum and urine.Recoveries were found to be in the range of 93.2%—97.3%,and the result of statistical calculation shows that relative standard deviations（RSDs） were lower than 4.08%.The results indicate that present method can be applied for the detection of DA in the human body.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2017年第12期2169-2175,共7页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:21575073)
山东省自然科学基金(批准号:ZR2013BQ004)
山东省高等学校科技计划项目(批准号:J15LC14)资助
关键词
金纳米簇
荧光
铜离子
多巴胺
Gold nanoclusters
Fluorescence
Copper ion
Dopamine
