摘要
本试验采用25 L自动发酵罐,在保持其他因素不变的前提下,通过调整发酵罐搅拌来控制发酵液的溶氧浓度。通过测定发酵过程中的溶氧浓度、菌体浓度、乙酸生成量、质粒稳定性及L-色氨酸(1)合成量等指标,确定了最适搅拌速度和搅拌桨大小。通过优化发酵搅拌条件,使得1的产量提高到了61.0 g/L。本研究为1的工业化生产优化提供了依据。
Under the premise of keeping other factors unchanged, agitator blade and agitation were adjusted to control dissolved oxygen concentration in fermentation broth in the 25 L fermentation tank for the fermentation of L-tryptophan （1）. The appropriate agitator blade size and stirring speed were confirmed with dissolved oxygen concentration, bacteria concentration, acetic acid concentration, stability of the plasmid and the yield of 1 during fermentation as the indicators. The optimized conditions provided appropriate dissolved oxygen concentration in the fermentation broth, and kept the producing strain a appropriate growth rate in the initial stage of fermentation. At the same time, the optimization measures kept the producing strain a higher growth rate and plasmid stability, with less acetic acid accumulation in the logarithmic growth phase. And the producing strain maintained a higher rate biosynthesis of 1 during the whole process of fermentation. Finally, the 1 production was increased to 61.0 g/L. Our work provides a basis for the industrial production of 1.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2017年第12期1738-1743,共6页
Chinese Journal of Pharmaceuticals
基金
国家"重大新药创制"科技重大专项(2012ZX09201101-008)
国家自然科学基金(81273413)
上海市自然科学基金(14ZR1440200)
关键词
搅拌
溶氧
质粒
L-色氨酸
agitation
dissolved oxygen concentration
plasmid
L-tryptophan
