摘要
为研究牛膝提取物对大鳞鲃Barbus capito生长性能、部分非特异性免疫指标和抗病性的影响,采用单因子试验方法,选取450尾体质量为(98.92±2.25)g的大鳞鲃,随机分成5组,分别投喂牛膝提取物水平为0(对照)、0.05%、0.10%、0.20%、0.40%的5种试验饲料,养殖周期为60 d。结果表明:在饲料中添加0.20%的牛膝提取物,能够显著提高大鳞鲃体质量、增重率和特定生长率;添加0.10%的牛膝提取物,可显著增加大鳞鲃的头肾体指数和脾体指数(P<0.05);与对照组相比,0.20%、0.40%牛膝提取物添加组大鳞鲃血清溶菌酶(LZM)和酸性磷酸酶(ACP)活力均有显著提高(P<0.05);用嗜水气单胞菌攻毒后,0.20%牛膝提取物添加组大鳞鲃存活率最高。研究表明,牛膝提取物可提高大鳞鲃生长性能、非特异性免疫力和抗病性,建议饲料中牛膝提取物的添加水平为0.20%。
A total of 450 individuals of bulatmai barbel Barbus capito with body weight of(98.92 ± 2.25) g were randomly allocated into a circulating water system and fed diets containing Achyranthes bidentata extracts(ABE) at a rate of 0%(a control group),0.05%,0.10%,0.20%,and 0.40% at water temperature of(25±1) ℃ for 60 d to investigate effects of ABE on growth performance,partial non-specific immunity and disease resistance of bulatmai barbel. The results showed that significantly higher body weight,weight gain ratio(WGR) and specific growth rate(SGR) were found in the fish fed the diets containing 0. 2% of ABE,and that significantly higher head kidney-somatic index and spleen-somatic index were observed in the fish fed the diets containing 0. 1% of ABE(P<0.05). There were significantly higher activities of acid phosphatase(ACP) and lysozyme(LZM) in the fish fed the diets containing 0.20% and 0.40% of ABE than in fish fed the control diet(P < 0.05). A challenge test of bulatmai barbel by pathogen Aeromonas hydrophila revealed that the maximal survival rate was observed in the infected fish fed the diet containing 0. 20% of ABE. The findings indicate that ABE leads to significantly promote growth performance,partial non-specific immunity and disease resistance of bulatmai barbell,with appropriate ABE content of 0.20%.
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2017年第6期676-681,共6页
Journal of Dalian Ocean University
基金
宿迁市农业科技自主创新资金项目(SQCX2017-02)
江苏省水产三新工程项目(Y2016-15)
关键词
牛膝
大鳞鲃
非特异性免疫
生长性能
Achyranthes bidentata
Barbus capito
non-specific immunity
growth performance
