摘要
目的:建立黄柏及黄柏炭饮片的HPLC特征图谱,分析黄柏饮片炒炭前后特征图谱的变化规律。方法:采用HPLC分析黄柏及黄柏炭饮片的特征图谱,色谱条件为ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈(A)-0.05 mol·L^(-1)磷酸二氢钾溶液(B)(磷酸调p H 3.4)梯度洗脱(0~5 min,5%~10%A;5~20 min,10%~14%A;20~50 min,14%~32%A;50~80 min,32%~70%A),检测波长215 nm,柱温35℃,流速1.0 m L·min^(-1),进样量10μL。结果:11批黄柏饮片中均标示出16个特征峰,并归属了其中的9个色谱峰,分别为绿原酸、隐绿原酸、盐酸黄柏碱、木兰花碱、盐酸药根碱、盐酸巴马汀、盐酸小檗碱、黄柏内酯、黄柏酮。通过对色谱峰的分析,黄柏炭仅能检出黄柏中的3个共有峰,且峰面积相差较大;另外标示出了2个特征峰,其中相对比例较高的特征峰经分析验证,确认其为盐酸小檗碱的加热分解产物——小檗红碱。结论:黄柏饮片炒炭前后的特征图谱存在显著差异。该方法准确可靠、重复性好,为黄柏和黄柏炭饮片的质量标准制定提供参考依据。
Objective： To establish the HPLC specific chromatograms of Phellodendri Chinensis Cortex decoction pieces and charred Phellodendri Chinensis Cortex decoction pieces,and to study on the change law of HPLC specific chromatograms of Phellodendri Chinensis Cortex before and after being processed. Method： HPLC was employed with the ZORBAX SB-C18 column, acetonitrile-0. 05 mol·L^-1 potassium dihydrogen phosphate solution（ phosphoric acid regulated pH to 3. 4） was employed as a mobile phase in gradient elution,the detection wavelength was 215 nm,column temperature was at 35 ℃ and the flow rate was 1. 0 m L·min^-1. Result： Eleven batches of Phellodendri Chinensis Cortex decoction pieces were marked with sixteen characteristic peaks, and chlorogenic acid,cryptochlorogenic acid, phellodendrine, magnoflorine, jatrorrhizine hydrochloride, palmatine hydrochloride,berberine hydrochloride, obaculatone and obakunone were assigned. Based on the analysis of characteristic peaks,only three common peaks were detected in charred Phellodendri Chinensis Cortex decoction pieces,and the area of these peaks was quite different; in addition,two feature peaks were identified,with the relative high proportion of the characteristic peak being analyzed and verified to be berberrabine,the product of the heat decomposition of berberine hydrochloride. Conclusion： There are significant differences in the specific chromatograms of Phellodendri Chinensis Cortex decoction pieces before and after being processed. The method is accurate and reproducible,which can provide the basis for the quality standard of Phellodendri Chinensis Cortex decoction pieces and charred Phellodendri Chinensis Cortex decoction pieces.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第24期41-45,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技基础性科研专项重点项目(2014FY111100)
关键词
黄柏
黄柏炭
饮片
特征图谱
绿原酸
隐绿原酸
黄柏酮
盐酸小檗碱
Phellodendri Chinensis Cortex
charred Phellodendri Chinensis Cortex
decoction pieces
specific chromatograms
chlorogenic acid
cryptochlorogenic acid
obakunone
berberine hydrochloride
