摘要
制备了半胱胺(CYS)自组装膜修饰的金电极传感器并用扫描电镜对传感器形貌进行了表征。利用差分脉冲伏安法(DPV)研究了5-羟色胺(5-HT)在此修饰电极上的电化学行为。实验发现,CYS自组装膜对5-HT的氧化能够起到明显的电催化作用。在此基础上考察了最佳检测条件,推导出了扩散系数等重要反应参数,发现了5-HT的反应机理。在5.0×10^(-7)~7.0×10^(-5)mol/L浓度范围内,5-HT的峰电流随着其浓度的增加而线性增加,检出限为2.8×10^(-8)mol/L。该电极重现性良好,性能稳定,可用于人体血样中5-HT的测定,回收率在88.6%~116%之间。
The self-assembled monolayer of cysteamine （CYS） was prepared and its morphology was characterized by scanning electron microscope （SEM）. The electrochemical behavior of 5-hydroxytryptamine （5-HT） at the CYS modified electrode was studied by different pulse vohammetry （DPV）. It was found the modified electrode showed an obvious electrocatalytic activity for the oxidation of 5-HT. Based on the above resuh, the optimum conditions were investigated and some important electrochemical parameters were calculated, such as diffusion coefficient. The reaction mechanism of 5-HT at the modified electrode was presented. The peak currents of 5-HT were linearly related to the concentrations in the range from 5.0×10-7 mol/L to 7.0×10-5 mol/L with the detection limit of 2.8×10-s mol/L. In addition, the CYS modified electrode showed good reproducibility and stability. The proposed method was applied to determine the concentration of 5-HT in the serum and the recovery was between 88.6% and 116%.
出处
《化学传感器》
CAS
2017年第3期47-52,共6页
Chemical Sensors
基金
国家自然科学基金项目(81402720)资助
河北省杰出青年基金项目(H2016206557)资助
关键词
半胱胺
金电极
五羟色胺
测定
cysteamine
gold electrode
5-hydroxytryptamine
determination
