摘要
为了探讨不同生境对绿盲蝽生长影响,从分子角度确定绿盲蝽在果园的适合生境。采用绿盲蝽专用诱芯调查了枣园、葡萄园的绿盲蝽发生量,并根据保守序列设计简并引物,克隆精氨酸激酶片段,荧光定量PCR检测了绿盲蝽成虫基因表达量。结果表明在枣园绿盲蝽的发生量始终高于葡萄园,枣园有3个发生高峰,而葡萄园有2个。测序结果表明目的序列含有783个核苷酸,可编码261个氨基酸。序列比对及进化树表明AL-AK与其他昆虫AK相比近似度85%以上,且同半翅目昆虫较为靠近。枣园绿盲蝽精氨酸激酶基因表达量低于葡萄园。以上结果说明枣园生境比葡萄园适合绿盲蝽的发育,为针对性的防治绿盲蝽提供理论基础。
This study aims to investigate the effects of different habitats on the growth of ApolyguslucorumMeyer-Dür, and to determine the suitable habitat of Apolyguslucorum Meyer-Dür in the orchard from themolecular level. We used special lure to investigate the occurrence content of Apolyguslucorum Meyer-Dür injujube orchard and vineyard. The primers were designed according to the conserved sequence, cloned thefragments of arginine kinase. The gene expression of Apolyguslucorum Meyer-Dür adult was detected byfluorescence quantitative PCR. The results showed that the occurrence content of Apolyguslucorum Meyer-Dürin jujube orchard was always higher than that in vineyard. There were three occurrence peaks in jujubeorchard, while two in vineyard. The sequencing results indicated that the target sequence contained 783 nucleotides and could encode 261 amino acids. Sequence alignment and phylogenetic tree indicated that AL-AK had a similarity of 85% or more compared with other insects, and was close to that of Hemiptera. Theexpression of arginine kinase gene in jujube orchard was lower than that in vineyard. The above results showedthat compared with vineyard, jujube habitat was more suitable for the development of Apolyguslucorum Meyer-Dür, which provided a theoretical basis for the control of Apolyguslucorum Meyer-Dür.
出处
《中国农学通报》
2017年第33期27-33,共7页
Chinese Agricultural Science Bulletin
基金
山西省农业科学院博士研究基金"绿盲蝽代谢及生殖相关基因研究"(YBSJJ1406)
山西省科技攻关项目"枣绿盲蝽综合防控技术研发与集成示范"(20150311015-5)
山西省科技重点研发项目子项目"枣树重大病虫害防控技术研发"(2015-TN-4-2)
关键词
绿盲蝽
精氨酸激酶
枣园
葡萄园
荧光定量
Apolygus lucorum Meyer-Dur
arginine kinase
jujube orchard
vineyard
real time fluorescence quantitative PCR
