摘要
目的观察蛞蝓有效提取物(EL-3)对宫颈癌He La细胞裸鼠移植瘤生长的影响。方法制备宫颈癌He La细胞裸鼠移植瘤模型,检查EL-3对瘤体积、瘤重及VEGF蛋白表达的影响,评估EL-3在体内治疗宫颈癌的有效性。结果 EL-3(10、30和100 mg/kg)实验组的瘤体积显著低于生理盐水组(P<0.05),同时,其移植瘤的瘤重抑制率分别为42.0%、67.0%和83.0%,提示EL-3在体内抗宫颈癌作用较强,且呈剂量依赖性;采用免疫组化检测证实EL-3下调宫颈癌裸鼠移植瘤细胞VEGF蛋白表达。结论 EL-3具有抑制宫颈癌He La细胞裸鼠移植瘤生长的作用,其机理可能与抑制肿瘤血管生成有关。
Objective To determine the effect of EL-3, an effective extraction of limax, on the growth of cervical cancer HeLa cell xenografts in nude mice. Methods The model of cervical cancer HeLa cells in nude mice was established, and the effect of EL-3 on the tumor volume, tumor weight and the expression of VEGF protein were examined.The effectiveness of EL-3 in the treatment of cervical cancer in vivo was evaluated. Results EL-3 can effectively inhibit the growth of human cervical cancer transplanted tumor in nude mice;tumor volume of 10,30,100 mg/kg dose group of EL-3 were significantly lower than that of normal saline group （P〈0.05） ;simultaneously, the transplanted tumor weight inhibitory rates were 42% ,67% and 83% respectively,suggesting that the effect of EL-3 in vivo against cervical cancer is stronger and in a dose-dependent manner.Immunohistoehemistry showed that EL-3 could down regulate the expression of VEGF protein of the transplanted tumor tissues of cervical cancer HeLa cells in nude mice. Conclusion EL-3 can inhibit the tumor growth of cervical cancer HeLa cells in nude mice, and the mechanism may be related to the inhibition of tumor angiogenesis.
出处
《中南医学科学杂志》
CAS
2017年第6期562-566,共5页
Medical Science Journal of Central South China
基金
湖南省教育厅科学研究项目(16C1407)
广州市科技计划项目(2003Z3-E5131)
衡阳市科技计划项目(2016KJ60)
关键词
宫颈癌
蛞蝓
蛞蝓有效提取物
HE
La细胞
治疗作用
cervical cancer
limax
effective extraction of limax
HeLa cells
therapeutical effect
