摘要
以国槐种子为原料,在单因素实验的基础上采用Box-Behnken(BB)和响应面确定国槐种子蛋白质的提取工艺,并建立国槐蛋白质提取工艺的数学模型,同时探讨蛋白质对DPPH和ABTS自由基的清除能力。结果表明,国槐种子蛋白质提取的最优工艺为pH8.2缓冲液包含0.82 mol/L的NaCl,超声提取29 min,料液比1∶31(g/mL),此条件下,国槐种子蛋白质的提取量为139.25 mg/g,理论预测值为139.36 mg/g,两者误差为0.08%,实验结果真实可靠。抗氧化实验表明,国槐种子蛋白质的质量浓度为38.04μg/mL时,对DPPH和ABTS自由基的清除率分别为29.44%和90.82%。国槐种子中含有较丰富的蛋白质,并对DPPH和ABTS自由基有清除作用,可以作为潜在天然抗氧化剂。
Based on the results of the single factor experiments, Box- Behnken （BB） design and response surface analysis was applied to investigate the effects of pH, ultrasound time, solid- liquid rate, NaC1 concentration on extraction yield of Sophora japonica L seed pretein. The quadratic term model of the protein extraction technology was established .Meanwhile, the antioxidant activity of protein on DPPH and ABTS free radical was evaluated. The results showed that the optimal extraction conditions of Sophora japonica L seed pretein were as follows：phosphate buffer with pH8.2 and containing 0.8 mol/L NaC1, ratio of liquid to solid of 31 mL/g, ultrasonic extraction time of about 29 min.Under this condition, the predicted extraction yield of protein from Sophora japonica L. was 139.36 mg,/g with a relative error of 0.08% when compared with the actual yield （139.25 mg/g） which proved the accuracy of the resuhs.When the protein mass concentration was 38.044 Ixg/mL, the scavenging rate on DPPH and ABTS free radical were 29.44% and 90.82% respectively. Sophora japonica L. abundantly contained seed proteins and the proteins had excellent free radical scavenging activity thus providing a good natural free radical scavenger
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第24期165-171,共7页
Science and Technology of Food Industry
基金
国家中医药管理局2012年中医药行业科研专项(201207002)
关键词
国槐
种子蛋白质
超声提取
响应面法
抗氧化
Sophora japonica L
seed protein
ultrasonic extraction
response surface methodology
antioxidant
