摘要
目的分析人类免疫缺陷病毒相关神经认知障碍(human immunodeficiency virus-related neurocognitive disorder,HAND)患者脑组织基因表达芯片数据的生物学网络调控及关键蛋白,为后期HAND预防及治疗提供新的理论依据。方法从基因芯片公共数据库(gene expression omnibus,GEO)中下载美国纽约HAND患者(14例)和非患者(7例)脑组织基因芯片数据,利用基因云,基因大数据分析(gene-cloud of biotechnology informs,GCBI)实验平台、Gen Clip 2.0和Sytoscape 3.5.1软件,筛选差异表达基因,探讨差异基因的蛋白交互作用网络和生物学通路,从转录组角度阐述HAND发展的分子机制。结果与对照组相比,HAND组共有780个(1.43%)差异表达基因;主要涉及免疫应答、免疫调控、抗感染、抗病毒、细胞信号传导及突触传递等功能。LYN和RPS4Y1基因为蛋白-蛋白交互作用网络的核心节点;DNM1、FLT1、NOTCH3、LYN、ISG15和RPS4Y1为关键表达基因,其中,DNM1、FLT1和NOTCH3基因在HAND组低表达,而LYN、ISG15和RPS4Y1基因为高表达,差异均具有统计学意义(均有P<0.05)。结论 HAND组和对照组有780个差异表达基因,LYN和RPS4Y1基因为蛋白网络核心节点,在HAND组中高表达,其功能主要涉及免疫应答、免疫调控、抗感染、抗病毒等生物学功能。
Objective To analyze the biological regulation and key proteins of brain tissue gene expression gene data in HIV-related neurocognitive disorder （HAND） patients, and to provide a new theoretical basis for HAND prevention and intervention. Methods Microarray gene chip data of brain tissue were downloaded from gene expression omnibus （GEO） public database including New York HAND patients （ld cases） and non-patients （7 cases）, and the differential expression genes were imported into the analysis software gene-cloud of biotechnology informs （GCBI） experimental, plat- form, GenClip 2. 0 and Sytoscape 3.5. 1. Exploring expression of protein interaction network, biological pathway, a^d the molecular mechanism of the development of HAND from the perspective of transcriptions. Results Compared with the con- trol group, there were 780 （ 1.43% ） differentially expressed genes between the two groups. The difference gene was mainly associated with immune response regulation, anti-infective, antiviral, cell signaling and synaptic transmission. LYN and RPS4Y1 genes are the key nodes of the protein-protein interaction network. DNM1, FLT1, NOTCH3, LYN, ISG15 and RPS4Y1 were the key expression genes. The DNM1, FLT1 and NOTCH3 genes were lower in the HAND group, while LYN, ISG15 and RPS4Y1 gene was highly expressed, and the difference was statistically significant （all P 〈0. 05）. Conclu- sions There were 780 differentially expressed genes in HAND patients and non-patients. LYN and RPS4Y1 genes are the core nodes of protein network and are highly expressed in brain tissue of HAND patients. The function mainly includes im- mune response, immune regulation, anti-infection, anti-virus and other biological functions.
出处
《中华疾病控制杂志》
CAS
CSCD
北大核心
2017年第12期1277-1281,共5页
Chinese Journal of Disease Control & Prevention
基金
陕西中医药大学2014年校内科研基金(14XJZR07)
四川省教育厅人文社科重点研究基地(SXJY1606)
关键词
HIV
基因表达
计算生物学
HIV
Gene expression
Computational biology
