摘要
为澄清芍药炭疽病致病菌种类及筛选高效抑菌剂,开展了本研究。首先从感病叶片中分离纯化了病原并开展了其致病力检测,然后对致病菌予以形态学鉴定及基于r DNA-ITS序列分析的分子鉴定,最后采用菌丝生长速率法研究了5种杀菌剂的化学抑制效果。研究结果表明,从感病叶片的病块组织中可分离到两种疑似病原菌,但只有一种致病。该致病菌孢子盘及分生孢子形态特征与已报道的胶孢炭疽菌(Colletotrichum gloeosporioides)形态特征一致;其5.8S r DNA及两侧ITS区序列与Gen Bank中序列号为HQ264179.1的同源性最高,为99%,确定其为胶孢炭疽菌(C.gloeosporioides)。
The study was carried out in order to clarify the pathogen causing anthracnose in peony（Paeonia lactiflora Pall.）and to screen the highly efficient fungicides against this disease. The fungi were first isolated and purified from the infectedleaves and the pathogenicity was detected by artificial inoculation method. Then the morphological characteristics andrDNA-ITS sequence of the pathogen causing anthracnose disease was studied. Finally, toxicity of five fungicides to thepathogen was tested using the methods of inhibiting mycelium growth. The results showed that two suspicious fungi wereisolated, but only one was confirmed pathogenic. The morphological characteristic of the acervulus and conidia from thepathogen was identical to Colletotrichum gloeosporioides. And the ITS 1-5.8S rDNA-ITS2 sequences of the pathogen werehighly homogenic with those of HQ264179.1 in GenBank database. Based on the above results, C. gloeosporioide wastherefore identified as the pathogen causing peony anthracnose.
出处
《山东农业大学学报（自然科学版）》
CSCD
2017年第6期906-910,共5页
Journal of Shandong Agricultural University：Natural Science Edition
基金
山东省自然科学基金(ZR2014CM028)
山东省农业重大应用技术创新项目(鲁财农指[2016]36号)
关键词
芍药
炭疽病
病原
RDNA-ITS
化学防治
Paeonia lactiflora Pall.
anthracnose
pathogen
rDNA-ITS
chemical control
