摘要
为探讨柯萨奇病毒B3 与克山病发病间的相关关系 ,先后采用特异性肠道病毒RTPCR、特异性B组柯萨奇病毒ELISA ,以及特异性柯萨奇病毒B3RTPCR对四川省冕宁县、喜德县、德昌县克山病病区潜慢型克山病患者以及健康人血中肠道病毒、B组柯萨奇病毒和柯萨奇病毒B3 分别进行检测。结果显示 :①肠道病毒在潜、慢型克山病组血标本中检出率很高 ,达 80 % ,与健康对照组差异显著 (P <0 0 1)。②血清CVB1～ 6IgM抗体阳性率明显高于健康对照组 (33%VS 0 % ,P <0 0 1) ;潜、慢型克山病组血清CVB1～ 6IgG抗体阳性率高于健康对照组 ,且差异显著 (2 3%VS 0 % ,P <0 0 1)。③柯萨奇病毒B3 潜、慢型克山病组的检出率仅为 10 % ,在B组柯萨奇病毒抗体IgM阳性病人中占 40 % (4 10 ) ,占肠道病毒阳性样本的 16 6 % (4 2 4)。认为病区潜、慢型克山病患者有肠道病毒感染存在 ,单独由柯萨奇病毒B3
To study the relationship between the infection of enteroviruses and the etiology of keshan disease, we examined the 30 blood samples from the latent - chronic KD patients from Mianning,Xider,Derchang county of Sichuan Province with LDE-PCR (long distance enterovirus-specific RTPCR), two sensitive and specific ELISAs (one forCVB 1-6 IgM and the other for CVB 1-6 IgG), and three-primer RTPCR specific for CVB 3. Results show that: 1) The infection rate of enteroviruses in the samples from the latent - chronic KD patients is higher than from the control group (80% VS 0%, P<0.01); 2)The CVB 1-6 antibody positive rate in latent-chronic KD is higher than that of the control group (IgM, 33% VS 0%,P<0.01; IgG, 23% VS 0%,P<0.01); 3) 16.6% (4/24)of enteroviruses positive samples or 40% (4/10)of CVB 1-6 IgM antibody positive samples can be identified as CVB 3. So, the infection of enter viruses maybe involves in the cause and development of latent-chronic keshan disease, and at least we conclude that the mutation of CVB 3 is not the only cause of KD.
出处
《卫生研究》
CAS
CSCD
北大核心
2002年第4期261-263,共3页
Journal of Hygiene Research
基金
卫生部基金 (No .98- 1 - 0 66)
