摘要
目的分析AFP阳性胃癌(alpha-fetoprotein positive gastric cancer,AFPGC)组织中胸苷酸合成酶(thymidylate synthase,TS)、胎盘型谷胱甘肽-S-转移酶(glutathione S-transferase Pi,GST-π)、P-糖蛋白(P-glycoprotein,P-gp)、DNA拓扑异构酶Ⅱ(topoisomeraseⅡ,TopoⅡ)的表达,并与AFP阴性胃癌(即普通胃癌)进行对比,以了解AFPGC耐药情况。方法采用免疫组织化学染色法(S-P法)检测临床病理已确诊的27例AFPGC及46例同期普通胃癌组织中TS、GST-π、P-gp、TopoⅡ四种蛋白抗原的表达情况。结果 TS、GST-π在AFPGC中的阳性表达率均高于其在普通胃癌中的阳性表达率(P<0.05),P-gp、TopoⅡ在两组胃癌中阳性表达率差异无统计学意义(P>0.05)。结论 AFPGC可能较普通胃癌更易出现多药耐药,多药耐药可能是AFPGC预后差的原因之一;AFP的表达可能为胃癌耐药性增加的危险因素之一,AFP可能成为间接评估胃癌患者对化疗药物的敏感性一项指标,以指导临床进行个体化治疗。
Objective To analyze the expressions of thymidylate synthase （TS）, glutathione S-transferase Pi （GST-π）, P-glycoprotein （P-gp） and topoisomerase IⅡI （Topo Ⅱ ） in alpha-fetoprotein positive gastric cancer （AFPGC）, in order to study the drug resistance of AFPGC. Methods Immunohistochemistry staining method （the S-P law） was used to examine the expressions of TS, GST-π, P-gp, Topo Ⅱ in 27 pathologically diagnosed as AFPGC and 46 pathologically diagnosed as alpha-fetoprotein negative gastric cancers （normal gastric cancer）. Results Compared with normal gastric cancer, expressions of TS and GST-π were higher in AFPGC （P 〈 0. 05） , but expressions of P-gp and Topo Ⅱ had no statistical difference between two groups （P 〉 0. 05）. Conclusion Compared with alpha-fetoprotein negative gastric cancer, AFPGC is more likely to be multidrug resistance （MDR） , MDR may be one reason of extremely poor prognosis of AFPGC. The expression of alpha-fetoprotein may be a risk factor for the increase of resistance of gastric cancer. Alpha-fetoprotein may be an indirect indication of the sensitivity of chemotherapy for gastric cancer, to guide the individualized treatment of gastric cancers.
出处
《胃肠病学和肝病学杂志》
CAS
2017年第12期1365-1369,共5页
Chinese Journal of Gastroenterology and Hepatology
