摘要
目的:建立同时测定活血促愈胶囊中人参皂苷Rg_1、人参皂苷Rb_1、三七皂苷R_1、积雪草苷、羟基积雪草苷含量的方法。方法:采用高效液相色谱法,使用Kromasil 100-5 C_(18)色谱柱,流动相为乙腈-水(梯度洗脱),流速1 m L·min^(–1),检测波长203 nm,柱温30℃。结果:人参皂苷Rg_1、人参皂苷Rb_1、三七皂苷R_1、积雪草苷、羟基积雪草苷阴性均无干扰;人参皂苷Rg_1、人参皂苷Rb_1、三七皂苷R_1、积雪草苷、羟基积雪草苷分别在0.702~7.02μg(r=0.999 6)、0.655 2~6.552μg(r=0.999 8)、0.135 6~1.356μg(r=0.999 9)、0.277 4~2.774μg(r=0.999 9)、0.638 4~6.384μg(r=0.999 9)范围内进样量与峰面积呈良好的线性关系;平均加样回收率(n=6)为97.1%~98.7%,RSD为1.5%~2.7%,3批样品中人参皂苷Rg_1、人参皂苷Rb_1、三七皂苷R_1、积雪草苷和羟基积雪草苷含量范围分别为4.11~4.27、3.82~3.94、0.72~0.84、1.71~1.88和3.10~3.19 mg·g^(-1)。结论:该方法可用于活血促愈胶囊中人参皂苷Rg_1、人参皂苷Rb_1、三七皂苷R_1、积雪草苷、羟基积雪草苷含量的同时测定。
Objective： To establish a method for simultaneous determination of ginsenoside Rgl, ginsenoside Rbl, notoginsenoside R1, asiaticoside and madecassoside in Huoxue Cuyu capsules. Methods： HPLC was performed on the column of Kromasill00-5 Cls with mobile phase of acetonitrile-water （gradient elution ）at a flow rate of 1 mL ~ min-1. The detection wavelength was 203 nm and the column temperature was 30℃. Results： No interference was found in negative samples. The linear ranges of ginsenoside Rg1, ginsenoside Rb1, notoginsenoside R1, asiaticoside and madecassoside were 0.702-7.02 μg（ r=0.999 6 ）, 0.655 2-6.552μg（ r=0.999 8 ）, 0.135 6-1.356μg （ r=0.999 9 ）, 0.277 4-2.774μg （ r=0.999 9 ） and 0.638 4-6.384μg （ r=0.999 9 ）, respectively. The average recovery rates were 97.1%-98.7% with RSDs between 1.5% and 2.7% （n=6）. The content ranges of ginsenoside Rgl, ginsenoside Rbl, notoginsenoside R1, asiaticoside and madecassoside in samples were 4.11-4.27 -1 -1 - -1 mg·g-1,3.82-3.94mg·g-1 ,0.72-0.84 mg·g-1, 1.71.88mg·g-1 and 3.10-3.19mg·g-1, respectively. Conclusion： The method can be used for the simultaneous determination of ginsenoside Rg1, ginsenoside Rbl, notoginsenoside RI, asiaticoside and madecassoside in Huoxue Cuyu capsules.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2017年第12期2185-2190,共6页
Chinese Journal of Pharmaceutical Analysis
基金
湖南省科技厅基金项目(06FJ3017)
湖南省中医药管理局基金项目(2008093)
关键词
人参皂苷
三七皂苷
积雪草苷
羟基积雪草苷
三七
积雪草
活血促愈胶囊
中成药成分测定
高效液相色谱法
ginsenoside
notoginsenoside
asiaticoside
madecassoside
Notoginseng Radix et Rhizoma
Centellae Herba
Huoxue Cuyu capsules
composition determination of Chinese patent medicine
HPLC
