摘要
目的:ELISA与HPLC法检测葛根素含量的相关性研究。方法:利用SPSS软件分析二者的相关性,并将数据进行线性拟合,得到相应的线性方程。结果:ELISA与HPLC法检测结果之间的P<0.01,相关系数为0.993,二者极显著相关;所得线性方程为Y=0.911X+0.852(R^2=0.9865),将ELISA结果带入方程可直接转化为药典法定HPLC检测结果。结论:通过二者相关性的研究,可使ELISA法与药典HPLC方法检测结果相互转化,其结果准确可靠,重复性好,可实现对药材及配方颗粒的高通量快速质量评价。
Objective： To research correlation of ELISA and HPLC on puerarin content detection. Methods： Analyse the correlation of the two methods by SPSS software, and linear fitting the data to get the corresponding linear equation. Results ： The P value between ELISA and HPLC is less than 0. 01, the correlation coefficient value is 0. 993, which shows significant correlation; The linear regression equation is Y =0.911X ＋ 0. 852 （R2 = 0.9865）, the results of ELISA can be directly translated into the pharmacopoeia of HPLC. Conclusion： Through the correlation research of ELISA and HPLC, the test results of ELISA and HPLC can mutually transformed, the results are accurate, reliable and with a good repeatability, which can realize the high-throughput fast quality evaluation of medicinal herbs and dispensing granules.
出处
《成都中医药大学学报》
2017年第4期20-22,共3页
Journal of Chengdu University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81473338
81274043)
北京中医药大学青年教师专项(2015-JYB-JSMS014)
