摘要
目的:建立多发性骨髓瘤分子生物学标本资料库,并探讨分子生物学研究中标本的采集、运送、保存和质量控制,以及标本库的信息化管理等相关问题。方法:收集多发性骨髓瘤患者骨髓标本及外周血标本。骨髓标本分离单个核细胞后进行磁珠分选浆细胞,分选所得的浆细胞分为2份,1份加适量TRIzol,编号后于-80℃低温冷冻保存用于后续RNA提取。另1份加适量小牛血清细胞冻存液,编号后于-80℃低温冷冻保存用于后续DNA提取;外周血标本分离血清、血浆,编号于-80℃低温冷冻保存。同时建立多发性骨髓瘤患者标本库的信息管理系统,实行专人管理并在使用过程中不断修改完善,以便于快速、有效的收集管理、查询及调用标本及临床资料数据。结果:已收集多发性骨髓瘤患者不同时期的浆细胞共244份,血清共564份,血浆1 005份。对所收集标本的临床资料及检查结果整理后归档管理。结论:初步建立了具有一定数量的多发性骨髓瘤分子生物学标本库,以保存骨髓分选的浆细胞为特色,为今后相关的多发性骨髓瘤分子生物学研究提供服务平台。
Objective： To establish a multiple myeloma specimen bank applied for molecular biological researches and to explore the methods of specimen collection,transportation,storage,quality control and the management of specimen bank.Methods： Bone marrow and blood samples were collected from multiple myeloma patients,plasma cell sorting were operated after the separation of mononuclear cells from bone marrow specimens. The plasma cells were divided into 2 parts,one was added with proper amount of TRIzol and then kept in -80 ℃ refrigerator for subsequent RNA extraction,the other was added with proper amount of calf serum cell frozen liquid and then kept in -80 ℃ refrigerator for subsequent cryopreservation of DNA extraction after numbered respectively. Serum and plasma were separated from peripheral blood,specimens of serum and plasma were then stored at -80 ℃ refrigerator after registration. Meantime,the myeloma specimen information management system was established,managed and maintained by specially-assigned persons and continuous modification and improvement in the process of use as to facilitate the rapid collection,management,query of the effective samples and clinical data.Results： A total of 244 portions plasma cells,564 portions of serum,and 1005 portions of plasma were collected,clinical characters were documented. Conclusion： A multiple myeloma specimen bank have been established initially,which can provide quality samples and related clinical information for molecular biological research on multiple myeloma.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2017年第6期1727-1732,共6页
Journal of Experimental Hematology
基金
南京总医院科研基金课题(2013051)
关键词
多发性骨髓瘤
标本库
分子生物学
浆细胞
multiple myeloma
specimen bank
molecular biology
plasma cell
