摘要
目的探讨黄芪甲苷、人参皂苷Rg1对慢性萎缩性胃炎大鼠Hedgehog信号通路的影响。方法雄性Wistar大鼠随机分为空白组、模型组、人参皂苷Rg1低剂量组、人参皂苷Rg1高剂量组、黄芪甲苷低剂量组、黄芪甲苷高剂量组、Purmorphamine组及叶酸组,运用幽门弹簧法制备萎缩性胃炎模型。应用HE染色评价胃黏膜病理损伤,应用Western Blot、RT-PCR和量子点介导的免疫荧光化学法分别检测各组大鼠胃组织Hedgehog信号通路的蛋白和基因表达水平。结果与空白组相比,模型组大鼠Shh、Ptch、Gli-1基因及蛋白含量均显著降低(P<0.05),SUFU蛋白表达明显升高(P<0.01),Cyclin D1表达减弱(P<0.01)。与模型组相比,人参皂苷Rg1低剂量组、人参皂苷Rg1高剂量组及叶酸组Shh蛋白表达明显升高(P<0.05),各给药组均能显著升高Ptch蛋白含量(P<0.05)。人参皂苷Rg1高剂量组、Purmorphamine组Gli-1蛋白表达明显升高(P<0.05),黄芪甲苷高剂量组、人参皂苷Rg1高剂量组和Purmorphamine组SUFU蛋白表达明显减弱(P<0.05)。黄芪甲苷高剂量组和Purmorphamine组Cyclin D1蛋白表达增强(P<0.05)。结论黄芪甲苷、人参皂苷Rg1对Hedgehog信号通路关键因子有一定的激活作用,进而改善慢性萎缩性胃炎大鼠的胃黏膜病变。
Objective To investigate the mechanism of astragaloside IV and ginsenoside Rg1 on hedgehog signal pathway in chronic atrophic gastritis （CAG）rats. Methods Male wistar rats were randomly divided into control group, model group, ginsenoside Rg1 low and high dose group, astragaloside IV low and high dose group, purmorphamine group and the folic acid group. Atrophic gastritis model was induced by implantation of a spring into the pylorus in combination with intragastric administration of hot salty starch paste. HE stain was used to examine the stomach injury. The mRNA and protein expression of hedgehog signal pathway was detected by RT-PCR, Western Blot analysis and immunofluorescence histochemistry. Results Compared with the control group, stomach injury in model group was obvious, the mRNA and protein expression of Shh, Ptch and Gli-1 was obviously down-regulated （ P〈0. 05 ） ; the expression of SUFU was increased and CyclinD1 was decreased （ P 〈 0.01 ）. Compared with the model group, the level of Shh was increased in the ginsenoside Rg1 low and high dose groups and the folic acid group（P〈0. 05）. The protein of Ptch was markedly up-regulated （ P〈0.05 ） in the treatment groups, and the expression of Gli-1 was significantly increased in ginsenoside Rg1 high dose group and the purmorphamine group. SUFU was down-regulated in Astragaloside IV high dose group, ginsenoside Rg1 high dose group and purmorphamine group （P〈0. 05）. The expression of CyclinD1 was up-regulated in astragaloside IV high dose group and purmorphamine group （P〈0. 05 ）. Conclusion Astragaloside Ⅳ, ginsenoside Rg1 can activate the key factors of hedgehog signaling pathway, and improve the gastric mucosal lesion in rats with chronic atrophic gastritis.
出处
《环球中医药》
CAS
2017年第12期1428-1433,共6页
Global Traditional Chinese Medicine
基金
国家自然科学基金(81373585)
