摘要
目的建立麻仁丸中芍药苷、橙皮苷、大黄素、和厚朴酚、厚朴酚和大黄酚的含量测定方法。方法采用反相高效液相色谱法(RP-HPLC),Waters Xbridge C_(18)色谱柱,流动相为乙腈-0.1%磷酸溶液,梯度洗脱,流速为1.0 mL/min,检测波长为230 nm(芍药苷),254 nm(大黄素、大黄酚),284 nm(橙皮苷),294nm(和厚朴酚、厚朴酚),柱温30℃。结果芍药苷、橙皮苷、大黄素、和厚朴酚、厚朴酚和大黄酚分别在0.39~61.06,0.46~60.24,0.15~30.50,0.20~60.41,0.26~60.76,0.16~30.71μg/mL范围内有良好的线性关系;平均回收率分别为98.4%,99.8%,97.7%,99.4%,98.8%和98.7%,RSD分别为1.16%,0.98%,1.43%,1.19%,1.78%和1.74%。结论该方法操作简便、结果准确、重复性好,可用于麻仁丸中芍药苷、橙皮苷、大黄素、和厚朴酚、厚朴酚和大黄酚含量的同时测定,可用于麻仁丸的质量控制。
Objective To establish a method for determination of paeoniflorin, hesperidin, emodin, honokiol, magnololand chrysophanol in Maren Pills. Methods A RP-HPLC method was established. The chromatographic column wasWaters Xbridge C18. Gradient elution was performed with acetonitrile-0.1 % phosphoric acid at a flow rate of 1.0mL/min. The detection wavelengths were 230 nm for paeoniflorin, 254 nm for emodin and chrysophanol, 284 nmfor hesperidin, 294 nm for magnolol and honokiol. The column temperature was 30 ℃. Results The linear ranges ofpaeoniflorin, hesperidin, emodin, honokiol, magnolol and chrysophanol were 0.39- 61.06, 0.46-60.24, 0.15-30.50,0.20-60.41, 0.26-60.76 and 0.16-30.71 μg/mL. The average recoveries were 98.4 %, 99.8 %, 97.7 %, 99.4 %, 98.8 %and 98.7 %, with RSDs of 1.16 %, 0.98 %, 1.43 %, 1.19 %, 1.78 % and 1.74 %, respectively. Conclusion The methodof is simple, accurate and reproducible, and can be used for the simultanious determination of paeoniflorin, hesperidin,emodin, honokiol, magnolol and chrysophanol in Maren Pills. It can be used for the quality control of Maren Pills.
出处
《食品与药品》
CAS
2017年第6期428-431,共4页
Food and Drug
