摘要
目的:研孕妇亚甲基四氢叶酸还原酶(MTHFR)基因C677T多态性与子痫前期的相关性。方法:选择2014年7月~2017年3月期间在建始县人民医院诊断为子痫前期的孕妇作为研究的PE组,同期在建始县人民医院产检并分娩的健康孕妇作为研究的对照组。测定外周血中MTHFR基因C677T位点的多态性以及同型半胱氨酸(Hcy)代谢指标的含量、凋亡基因及侵袭基因的表达量。结果:PE组孕妇外周血中MTHFR基因C677T位点TT基因型的比例显著高于对照组,CT及CC基因型的比例均均显著低于对照组;TT基因型PE孕妇血清及胎盘中Hcy的含量以及胎盘中FasL、Caspase-8、Bax、Caspase-9、Caspase-3的mRNA表达量显著高于CT基因型及CC基因型,血清及胎盘中叶酸的含量以及胎盘中Notch-1、N-cadherin、Vimentin、CatL、CatB的mRNA表达量显著低于CT基因型及CC基因型PE孕妇。结论:MTHFR基因C677T位点突变能够通过影响Hcy代谢以及凋亡基因、侵袭基因表达的方式参与子痫前期的发生。
Objective:To study the correlation between methylenetetrahydrofolate reductase(MTHFR)gene C677 T polymorphism and preeclampsia in pregnant women.Methods:Pregnant women who were diagnosed with preeclampsia in Jianshi People's Hospital between July 2014 and March 2017 were selected as the PE group of the research,and healthy pregnant women who received antenatal care and gave birth in Jianshi People's Hospital during the same period were selected as the control group of the research.The MTHFR gene C677 Tpolymorphism in peripheral blood,the contents of homocysteine(Hcy)metabolism indexes and the expression of apoptosis genes and invasion genes were determined.Results:The proportion of MTHFR gene C677 Tlocus TT genotype in peripheral blood of PE group was significantly higher than that of control group while the proportion of CT and CC genotypes were significantly lower than those of control group;Hcy levels in serum and placenta as well as FasL,Caspase-8,Bax,Caspase-9 and Caspase-3 mRNA expression in placenta of PE women with TT genotype were significantly higher than those of PE women with CT genotype and CC genotype while folic acid levels in serum and placenta as well as Notch-1,N-cadherin,Vimentin,CatL and CatB mRNA expression in placenta were significantly lower than those of PE women with CT genotype and CC genotype.Conclusions:MTHFR gene C677 Tlocus mutation can participate in the occurrence of preeclampsia by affecting the Hcy metabolism as well as the expression of apoptosis genes and invasion genes.
出处
《海南医学院学报》
CAS
2017年第22期3054-3057,共4页
Journal of Hainan Medical University
基金
湖北省教育厅科学研究中青年人才项目(Q20151811)~~
