摘要
采用气相色谱技术分析了沙棘果油与沙棘籽油的脂肪酸组成,并利用清除1,1-二苯基-2-苦肼基自由基(DPPH·)法与羟自由基(·OH)法评价了沙棘果油和沙棘籽油的抗氧化活性。结果表明:沙棘果油主要含棕榈油酸(35.945%)、棕榈酸(34.108%)、油酸(18.357%)和亚油酸(6.198%),不饱和脂肪酸含量为62.501%;沙棘籽油主要含亚油酸(38.958%)、亚麻酸(29.327%)、油酸(20.859%)和棕榈酸(7.659%),不饱和脂肪酸含量为89.965%;沙棘果油、沙棘籽油和BHT清除DPPH·的IC50分别为25.68、17.29μg/mL和27.61μg/mL;清除·OH的IC50分别为29.32、25.48μg/mL和33.60μg/mL;表明沙棘籽油与沙棘果油均具有较强的抗氧化活性,且沙棘籽油的抗氧化活性强于沙棘果油。
Fatty acid compositions of sea buckthorn pulp oil and sea buckthorn seed oil were analyzed by gas chromatography and the antioxidant activities of sea buckthorn pulp oil and sea buckthorn seed oil were evaluated by scavenging 1,1-diphenyl-2- picrylhydrazyl hydrate free radical （DPPH·） and hydroxyl free radical（·OH）. The results showed that sea buckthorn pulp oil mainly contained palmitoleic acid （35.945%）, palmitic acid （34.108%）, oleic acid （18.357%） and linoleic acid （6.198%）, and the content of unsaturated fatty acid was 62.501%. Sea buckthorn seed oil mainly contained linoleic acid （38.958%）, linolenic acid （29.327%）, oleic acid （20.859%） and palmitic acid （7.659%）, and the content of unsaturated fatty acid was 89.965%. The IC50 of sea buckthorn pulp oil, sea buckthorn seed oil and BHT were 25.68, 17.29 μg/mL and 27.61 μg/mL respectively for scavenging DPPH·, and the IC50 were 29.32, 25.48 μg/mL and 33.60 μg/mL respectively for scavenging ·OH, which indicated that sea buckthorn pulp oil and sea buckthorn seed oil had strong antioxidant activities, and the antioxidant activity of sea buckthorn seed oil was stronger than that of sea buckthorn pulp oil.
出处
《中国油脂》
CAS
CSCD
北大核心
2017年第12期120-123,共4页
China Oils and Fats
基金
国家级大学生创新创业训练计划项目(201610124001)
山西省1331工程重点建设计划支持项目(2017)
山西省高等学校大学生创新创业训练计划项目(2016381)
忻州师范学院重点学科项目(XK201502)
忻州师范学院化学化工创新基地项目(2017)
关键词
沙棘籽油
沙棘果油
脂肪酸组成
抗氧化活性
sea buckthorn pulp oil
sea buckthorn seed oil
fatty acid composition
antioxidant activity
