摘要
利用选择性培养基、人工胃液及含胆盐培养基,从如皋长寿地区的人群肠道中分离筛选出耐酸耐胆盐能力较强的乳酸菌,并研究其降胆固醇能力及方式。结果表明,分离筛选的60株乳酸菌在pH 3.0的人工胃液中存活率均>30%;来源于中年组、老年组及长寿组的乳酸菌存活率分别为69%,71%,70%,均显著高于青少年组(P<0.05);在0.3%及0.5%胆盐培养基中的存活率均>20%,老年组及长寿组的乳酸菌在0.3%胆盐培养基中的存活率分别为53.85%,55.26%,均显著高于青少年组和中年组(P<0.05);来源于长寿组的乳酸菌降胆固醇能力显著高于中年组(P<0.05),其中,乳杆菌属与魏斯氏菌属的胆固醇降解率显著高于肠球菌属(P<0.05),其降胆固醇的主要方式为细胞吸收和共沉淀。来源于长寿地区人群的乳酸菌耐酸耐胆盐能力及降胆固醇能力较强,其中长寿组的乳酸菌要高于其他年龄组。
Lactic acid bacteria （LAB） with ability of acid and bile salt resistance were isolated from the feces of the longevity population in Rugao by using selective medium, artificial gastric juice and bile salt medium, and the ability of cholesterol reducing and manner were studied. The results showed that the survival rate of all isolated 60 strains of LAB in the pH 3.0 artificial gastric juice was more than 30%, the LAB from the middle-aged group, elderly group and lon gevity group was significantly higher than that in the youth group （P〈0.05）; the survival rate of LAB in the 0.3% and 0.5% bile salt culture medium was more than 20%, of which the LAB in elderly group and longevity group was significantly higher than that of youth group and middle-aged group （P〈0.05）. The ability of cholesterol lowering of the LAB from the longevity group was significantly higher than that of middle aged group （P＇Q0.05）, the ability of cho-lesterol reducing of tactobacillus spp. and Weissella spp. was signifi candy higher than that of Enterococcus spp. （P〈0.0,5）, the main manners of cholesterol lowering of LAB were cell absorption and sedimentation. The LAB come from longevity population have the strong ability of acid and bile salt resistance and cholesterol lowering, and the longevity group are higher than others from other age groups.
出处
《食品与机械》
CSCD
北大核心
2017年第10期1-5,共5页
Food and Machinery
基金
国家自然科学基金青年基金项目(编号:31701627)
国家自然科学基金基金面上项目(编号:31571855)
江苏省高校自然科学研究面上项目(编号:17KJB550009)
江苏省高校自然科学研究重大项目(编号:17KJA550004)
江苏省乳品生物技术与安全控制重点实验室开放课题(编号:K14003)
关键词
乳酸菌
降胆固醇
耐酸耐胆盐
长寿地区
年龄组
longevity area
age groups
Lactic acid bacteria
acid and bile salt resistanee
cholesterol lowering
