摘要
单级自养脱氮(completely autotrophic nitrogen removal over nitrite,CANON)存在启动周期长、运行易失稳的问题.本文研究了在淹没式生物滤池(submerged biological aerated filter,SBAF)体系内,采用前期更替曝气的满负荷启动方法,通过严格控制体系中的溶解氧(dissolved oxygen,DO)和温度(DO和温度分别控制在0.10~0.30 mg·L^(-1)和31℃±1℃的范围内),水力停留时间(hydraulic retention time,HRT)控制为24 h,于51 d成功实现CANON的快速启动,并稳定运行278 d.该体系氨氮去除率(ammonium removal rate,ARR)最大值为98.9%,其平均值为95.1%;总氮去除率(total nitrogen removal rate,TNR)最大值为85.9%,其平均值为75.1%;体系存在少量NO_3^--N累积.采用16S rDNA宏基因组高通量测序技术分析该体系中污泥微生物的演化特征,发现起亚硝化作用的富集微生物主要为门Proteobacteria,起厌氧氨氧化作用的富集微生物主要为门Planctomycete属Candidatus Brocadia,其相对丰度随着驯化时间延长而逐渐增大,两者协同作用共同实现CANON中的总氮去除.
The CANON process has the disadvantages of long start-up periods and unstable operation. In the SBAF system, under strictly controlled conditions of dissolved oxygen （DO） and temperature, a CANON process is started up in 51 days and is operated stably for 278 days using a new method of independent research and development. The results show that the maximum and average ARR are 98. 9% and 95. 1% , respectively. The maximum and average TNR are 85. 9% and 75. 1% , respectively. Furthermore, a small quantity of nitratenitrogen exists in this system. The microbial structure features of the sludge are analyzed by 16S rDNA macro high-throughput genome sequencing. It is verified that Proteobacteria is the main microorganism in the AOB, and Planctomycete Candidatus Brocadia is the main microorganism in the AnAOB. Both of them collaborate on total nitrogen removal in the CANON process.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2017年第12期5192-5200,共9页
Environmental Science
基金
广东省科技计划项目(2016B090918036)
广州市科技计划项目(201510010199)
