摘要
用含有沉积物的氮化物污染实际水体作为测定体系,通过测定水体无机三态氮、总氮、COD、pH以及沉积物总氮、COD和对4种代谢酶活性的动力学过程的监测,评价了一株不产氧光合细菌——海洋着色菌(Marichromatium gracile)YL28对海水实际养殖水体脱氮效应。结果表明:水体本身具有一定的自净作用;与对照组相比,YL28组水体氨氮、亚硝氮、硝氮、沉积物总氮和COD的残留率明显降低(P<0.05),在6~10d内水体COD明显升高,水体总氮和pH未见显著差异。处理过程中,在特定的时间范围内,转化酶、蛋白酶、脲酶和脱氢酶活性明显升高(P<0.05),与沉积物总氮和COD去除规律相吻合。由此可见,添加YL28对污染海水养殖水体中无机三态氮、沉积物有机氮化物和有机碳化物具有明显的去除作用,本研究为海水养殖微生态水质修复剂的开发和应用奠定基础。
The actual mariculture nitrogen pollution water containing sediments was selected as the measurement system. Effects of a anoxygenic phototrophic bacterium Marichromatiurn gracile YL28 on the denitrification of actual mariculture water were studied by the method of kinetic process about inorganic nitrogen, total nitrogen （TN）, COD and pH in overlying water, as well as TN, COD and metabolic activity of the four enzymes in sediment. Results showed that the water body itself had a certain self-purification effect; compared with control system, the residual rate of ammonia, nitrate and nitrite in water, TN and COD in sediment significantly decreased （P〈0.05） in the YL28 system. The COD of YL28 system increased significantly in 6-10 days, and TN and pH were no significant difference. The activity of invertase, protease, urease and dehydrogenase increased significantly （P〈0.05） in the specific time range of the process, which was consistent with the removal of TN and COD. It indicates that there is obvious removing effect for inorganic nitrogen in contaminated mariculture water and organic nitrides and carbides in sediment by adding YL28. This study lays the foundation for the development and application of micro-ecological water quality restorer preparation for mariculture.
出处
《生物资源》
CAS
2017年第6期441-447,共7页
Biotic Resources
基金
国家海洋公益性行业科研专项项目(No.201505026)
华侨大学研究生科研创新能力培育计划资助项目
关键词
不产氧光合细菌
海洋着色菌
水质修复剂
脱氮
anoxygenic phototrophic bacterium
Marichrornatium gracile
water quality restorer
nitrogen removal
