摘要
以大田毛建草为试验材料,通过研究不同外植体、不同激素种类和浓度配比对再生芽诱导、增殖培养及不定根诱导的影响,首次建立了毛建草离体培养快繁体系。结果表明:带腋芽茎段是毛建草组培快繁最适外植体;芽诱导培养基为MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+IAA 0.2 mg/L,诱导率为63.6%;继代培养基为MS+6-BA 0.75 mg/L+KT 4.0 mg/L+NAA 0.01 mg/L+GA30.2 mg/L,平均增殖数为4.3;不定根诱导培养基为MS+NAA 0.2 mg/L,生根率为85.1%;试管苗移栽成活率为100%,研究结果为毛建草组培苗的工厂化繁育奠定了基础。
In this study, in vitro culture system of Dracocephalum rupestre Hance was established for the first time by evaluating the effects of different explants, different types and concentrations of plant hormones on bud induction,proliferation and adventitious root induction. The results indicated that the stem with axillary buds was the most suitable explants for Dracocephalum rupestre Hance tissue culture and rapid propagation; the optimum medium for bud induction was MS ＋6-BA 0.5 mg/L ＋NAA 0.1 mg/L ＋IAA 0.2 mg/L, and the average induction rate was 63.6%; the proliferation culture medium for bud differentiation was MS＋6-BA 0.75 mg/L＋KT 4.0 mg/L＋NAA 0.01 mg/L＋ GA3 0.2 mg/L, with the average single bud proliferation number being 4.3; the optimum adventitious root induction medium was MS＋NAA 0.2 mg/L, and the rooting rate was 85.1%; the transplanting survival rate reached 100%. The results laid the foundation for the industrial production of Dracocephalum rupestre Hance plantlets.
出处
《北方农业学报》
2017年第6期102-106,共5页
Journal of Northern Agriculture
基金
内蒙古自然科学基金项目(2015MS0386)
关键词
毛建草
组织培养
快速繁殖
Dracocephalum rupestre Hance
Tissue culture
Rapid propagation
